All lanes : Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459) at 1/10000 dilutionLane 1 : HeLa cell lysates untreated with alkaline phosphatase Lane 2 : HeLa cell lysates treated with alkaline phosphatase Lysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution
ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
ab109459 at 1/100 dilution staining MCM2 (phospho S27) in HeLa cells by Immunofluorescence.