MCM2

Gene Symbol MCM2
Entrez Gene 4171
Alt Symbol BM28, CCNL1, CDCL1, D3S3194, MITOTIN, cdc19
Species Human
Gene Type protein-coding
Description minichromosome maintenance complex component 2
Other Description DNA replication licensing factor MCM2|MCM2 minichromosome maintenance deficient 2, mitotin|cell devision cycle-like 1|cyclin-like 1|minichromosome maintenance deficient 2 (mitotin)|minichromosome maintenance protein 2 homolog|nuclear protein BM28
Swissprots P49736 Q15023 Q96AE1 Q14577 Q9BRM7 Q969W7 Q8N2V1
Accessions AAT70723 CAR95152 CBX85462 CCD32687 EAW79339 P49736 AK128291 BAG54656 AK299934 BAG61767 AK304204 BAH14132 AK309335 BC000300 AAH00300 BC006165 AAH06165 BC007670 AAH07670 BC007938 AAH07938 BC014272 AAH14272 BC017258 AAH17258 BC017490 AAH17490 BC030131 AAH30131 BM153020 BT009733 AAP88735 BT009734 AAP88736 D21063 BAA04642 D83987 BAA12177 DB100427 X67334 CAA47749 NM_004526 NP_004517 NR_073375
Function Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. {ECO:0000269|PubMed:8175912}.
Subcellular Location Nucleus {ECO:0000269|PubMed:8175912}.
Top Pathways DNA replication, Cell cycle

Phospho-MCM2 (Ser139) (D1Z8X) XP ® Rabbit mAb - 12958 from Cell Signaling Technology

Prices $318.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1Z8X
Western blot analysis of HeLa cell extracts, untreated (-) or calf intestinal phosphatase (CIP) and λ phosphatase-treated (+), and extracts of T98G cells serum-starved for 48 hours (-) or serum starved for 48 hours followed by serum-stimulation for 24 hours (+), using Phospho-MCM2 (Ser139) (D1Z8X) XP ® Rabbit mAb (upper), MCM2 (D7G11) XP ® Rabbit mAb #3619 or α-Actinin (D6F6) XP ® Rabbit mAb #6487.

Phospho-MCM2 (Ser139) Antibody - 8861 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from WI-38 cells, synchronized by serum starvation, using Phospho-MCM2 (Ser139) Antibody (upper), MCM2 (D7G11) XP ® Rabbit mAb #3619 (middle), and β-Actin (13E5) Rabbit mAb #4970 (lower). Cells were synchronized for 72 hours and then released by addition of serum and harvested at the times indicated.

MCM2 (D7G11) XP ® Rabbit mAb - 3619 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7G11
Western blot analysis of extracts from various cell types using MCM2 (D7G11) XP ® Rabbit mAb.

MCM2 Antibody - 4007 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using MCM2 Antibody.

MCM2 (1E7) Mouse mAb - 12079 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1E7
Western blot analysis of extracts from various cell lines using MCM2 (1E7) Mouse mAb.

MCM2 (E-8) - sc-373702 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MCM2 (E-8): sc-373702. Western blot analysis of MCM2 expression in HeLa (A) and K-562 (B) nuclear extracts.

MCM2 (N-19) - sc-9839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MCM2 (N-19): sc-9839. Western blot analysis of MCM2 expression in HeLa nuclear extract.

MCM2 (H-126) - sc-10771 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MCM2 (H-126): sc-10771. Western blot analysis of MCM2 expression in K-562 (A), NIH/3T3 (B) and MCF7 (C) nuclear extracts.

MCM2 (SPM196) - sc-56322 from Santa Cruz Biotechnology

Prices $279.00
Sizes 250 µl supernatant
Host Mouse
Clonality Monoclonal
MCM2 (SPM196): sc-56322. Western blot analysis of MCM2 expression in HeLa (A), MEG-01 (B), CCRF-CEM (C), MOLT-4 (D), Ramos (E) and DU 145 (F) nuclear extracts.

MCM2 (CRCT2.1) - sc-56321 from Santa Cruz Biotechnology

Prices $279.00
Sizes 250 µl supernatant
Host Mouse
Clonality Monoclonal
MCM2 (CRCT2.1): sc-56321. Western blot analysis of MCM2 expression in HeLa (A), MEG-01 (B), CCRF-CEM (C), MOLT-4 (D), Ramos (E) and DU 145 (F) nuclear extracts.

MCM2 (D1.9H5) - sc-73572 from Santa Cruz Biotechnology

Prices $279.00
Sizes 250 µl supernatant
Host Mouse
Clonality Monoclonal
MCM2 (D1.9H5): sc-73572. Western blot analysis of MCM2 expression in HeLa (A), MEG-01 (B), CCRF-CEM (C), MOLT-4 (D), Ramos (E) and DU 145 (F) nuclear extracts.

Anti-MCM2 (phospho S108) antibody [EPR4121] - ab109271 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4121
All lanes : Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/1000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate, treated with Alkaline Phosphatase (AP) Lysates/proteins at 10 µg per lane.

Anti-MCM2 (phospho S139) antibody [EPR10819] - ab170873 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10819
ab170873 showing +ve staining in Human normal colon tissue.

Anti-MCM2 (phospho S27) antibody - ab11896 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM2 (phospho S27) with ab11896 at 1/1000 (1µg/ml) and 1/5000 (0.2µg/ml). Detection: DAB.

Anti-MCM2 (phospho S27) antibody - ab84142 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84142, at 1/250 dilution, staining MCM2 in formalin-fixed, paraffin-embedded serial sections of human colon carcinoma by Immunohistochemistry. Mock phosphatase treated section (left) CIP phosphatase treated section (right). Detection: DAB staining using anti-Rabbit HRP conjugated antibody at 1/100.

Anti-MCM2 (phospho S27) antibody [EPR4119] - ab109459 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4119
All lanes : Anti-MCM2 (phospho S27) antibody [EPR4119] (ab109459) at 1/10000 dilutionLane 1 : HeLa cell lysates untreated with alkaline phosphatase Lane 2 : HeLa cell lysates treated with alkaline phosphatase Lysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution

Anti-MCM2 (phospho S32) antibody - ab11897 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryengeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM2 (phospho S32) with ab11897 at 1/1000 (1µg/ml). Detection: DAB.

Anti-MCM2 (phospho S40 + S41) antibody - ab70371 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right) tissues labelling MCM2 (phospho S40 + S41) with ab70371 at 1/200 (1µg/ml). Detection: DAB.

Anti-MCM2 (phospho S40 + S41) antibody - ab84391 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84391, at a 1/250 dilution, staining MCM2 (phospho S40 + S41) in formalin fixed, paraffin embedded human colon adenocarcinoma tissue by Immunohistochemistry. 1) Mock phosphatase treated section 2) Calf intestinal phosphatase-treated section Detection: DAB staining.

Anti-MCM2 (phospho S40) antibody [EPR4170(2)] - ab133243 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4170(2)
All lanes : Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (ab133243) at 1/1000 dilutionLane 1 : HeLa cell lysate untreatedLane 2 : HeLa cell lysate alkaline phosphatase treatedLysates/proteins at 10 µg per lane.

Anti-MCM2 (phospho S41) antibody [EPR4171] - ab109270 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4171
All lanes : Anti-MCM2 (phospho S41) antibody [EPR4171] (ab109270) at 1/50000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate, treated with Alkaline Phosphatase (AP)Lysates/proteins at 10 µg per lane.

Anti-MCM2 (phospho S53) antibody - ab70367 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM2 (phospho S53) with ab70367 at 1/200 (1µg/ml). Detection: DAB.

Anti-MCM2 (phospho S53) antibody - ab83969 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab83969, at a 1/500 dilution, staining MCM2 (phospho S53) in formaldehyde fixed, paraffin embedded human colon adenocarcinoma sections by Immunohistochemistry. Left image: mock phosphatase treated section Right image: calf intestinal phosphatase treated section. Detection: DAB staining using anti-rabbit IHC antibody at a dilution of 1/100.

Anti-MCM2 (phospho S53) antibody [EP4120] - ab109133 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP4120
All lanes : Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133) at 1/10000 dilutionLane 1 : HeLa cell lysate untreated Lane 2 : HeLa cell lysate treated with alkaline phosphatase (AP)Lysates/proteins at 10 µg per lane.

Anti-MCM2 antibody - ab4461 from Abcam

Prices $401.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling MCM2 with ab4461 at 1/1000 (1µg/ml). Detection: DAB.
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