Anti-MCM2 antibody
| Name | Anti-MCM2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab4461 |
| Prices | $401.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF IHC-P WB IP ICC/IF |
| Species Reactivities | Mouse, Chicken, Human, Xenopus, Chimpanzee, Ape, Orangutan |
| Antigen | Synthetic peptide corresponding to Human MCM2 |
| Description | Rabbit Polyclonal |
| Gene | MCM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling MCM2 with ab4461 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-MCM2 antibody (ab4461) at 1 µg/mlLane 1 : NIH3T3 whole cell lysateLane 2 : TCMK-1 whole cell lysateLane 3 : 4T1 whole cell lysateLane 4 : CT26 whole cell lysateLysates/proteins at 50 µg per lane.
ICC/IF image of ab4461 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4461, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ab4461 staining MCM2 in Human placenta.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Progenitor cell dynamics in the Newt Telencephalon during homeostasis and - Progenitor cell dynamics in the Newt Telencephalon during homeostasis and
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A role for DNA polymerase theta in the timing of DNA replication. - A role for DNA polymerase theta in the timing of DNA replication.
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Transcriptomic analysis of tail regeneration in the lizard Anolis carolinensis - Transcriptomic analysis of tail regeneration in the lizard Anolis carolinensis
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The DNA translocase FANCM/MHF promotes replication traverse of DNA interstrand - The DNA translocase FANCM/MHF promotes replication traverse of DNA interstrand
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MCM-BP regulates unloading of the MCM2-7 helicase in late S phase. - MCM-BP regulates unloading of the MCM2-7 helicase in late S phase.
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Efficient regeneration by activation of neurogenesis in homeostatically quiescent - Efficient regeneration by activation of neurogenesis in homeostatically quiescent
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XRCC1 interacts with the p58 subunit of DNA Pol alpha-primase and may coordinate - XRCC1 interacts with the p58 subunit of DNA Pol alpha-primase and may coordinate
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The interaction profile of homologous recombination repair proteins RAD51C, - The interaction profile of homologous recombination repair proteins RAD51C,
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Human TopBP1 participates in cyclin E/CDK2 activation and preinitiation complex - Human TopBP1 participates in cyclin E/CDK2 activation and preinitiation complex
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An essential role for Cdk1 in S phase control is revealed via chemical genetics - An essential role for Cdk1 in S phase control is revealed via chemical genetics
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