Anti-MCM4 antibody [EPR5247(2)]

• Supplier: Abcam
• Catalog: ab188578
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5247(2)
• Applications: FC IP ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MCM4 aa 1-100 (phospho S54)
• Description: Rabbit Monoclonal
• Gene: MCM4
• Conjugate: Unconjugated

Product images

All lanes : Anti-MCM4 antibody [EPR5247(2)] (ab188578) at 1/5000 dilutionLane 1 : 293 cell lysateLane 2 : Jurkat cell lysateLane 3 : K562 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

All lanes : Anti-MCM4 antibody [EPR5247(2)] (ab188578) at 1/5000 dilutionLane 1 : C6 cell lysateLane 2 : Raw 264.7 cell lysateLane 3 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling MCM4 with ab188578 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MCM4 with ab188578 at 1/40 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot analysis of MCM4 in 293 cell lysate immunoprecipitated with ab188578 at 1/50 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.