Anti-MCM5 antibody
| Name | Anti-MCM5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab17967 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human, Xenopus, Chimpanzee, Orangutan |
| Antigen | Synthetic peptide representing a portion of human minichromosome maintenance deficient 5 encoded within exon 2 |
| Description | Rabbit Polyclonal |
| Gene | MCM5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM5 with ab17967 at 1/1000 (1µg/ml). Detection: DAB.
![MCM5 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MCM5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17967.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 97kDa; MCM5](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/20507_ab17967-207984-IPVI003ab179674m.jpg)
MCM5 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MCM5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17967.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 97kDa; MCM5
ICC/IF image of ab17967 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Immortalised breast epithelia survive prolonged DNA replication stress and return - Immortalised breast epithelia survive prolonged DNA replication stress and return
Maya-Mendoza A, Merchut-Maya JM, Bartkova J, Bartek J, Streuli CH, Jackson DA. Cell Death Dis. 2014 Jul 24;5:e1351.
The DNA translocase FANCM/MHF promotes replication traverse of DNA interstrand - The DNA translocase FANCM/MHF promotes replication traverse of DNA interstrand
Huang J, Liu S, Bellani MA, Thazhathveetil AK, Ling C, de Winter JP, Wang Y, Wang W, Seidman MM. Mol Cell. 2013 Nov 7;52(3):434-46.
MCM-BP regulates unloading of the MCM2-7 helicase in late S phase. - MCM-BP regulates unloading of the MCM2-7 helicase in late S phase.
Nishiyama A, Frappier L, Mechali M. Genes Dev. 2011 Jan 15;25(2):165-75.
MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits - MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits
Afanasyeva EA, Mestdagh P, Kumps C, Vandesompele J, Ehemann V, Theissen J, Fischer M, Zapatka M, Brors B, Savelyeva L, Sagulenko V, Speleman F, Schwab M, Westermann F. Cell Death Differ. 2011 Jun;18(6):974-84.
A reduction of licensed origins reveals strain-specific replication dynamics in - A reduction of licensed origins reveals strain-specific replication dynamics in
Kawabata T, Yamaguchi S, Buske T, Luebben SW, Wallace M, Matise I, Schimenti JC, Shima N. Mamm Genome. 2011 Oct;22(9-10):506-17.
The cyclin A centrosomal localization sequence recruits MCM5 and Orc1 to regulate - The cyclin A centrosomal localization sequence recruits MCM5 and Orc1 to regulate
Ferguson RL, Pascreau G, Maller JL. J Cell Sci. 2010 Aug 15;123(Pt 16):2743-9.
Cyclin E-dependent localization of MCM5 regulates centrosome duplication. - Cyclin E-dependent localization of MCM5 regulates centrosome duplication.
Ferguson RL, Maller JL. J Cell Sci. 2008 Oct 1;121(Pt 19):3224-32.
TBX5 is required for embryonic cardiac cell cycle progression. - TBX5 is required for embryonic cardiac cell cycle progression.
Goetz SC, Brown DD, Conlon FL. Development. 2006 Jul;133(13):2575-84. Epub 2006 May 25.