Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either normal rabbit IgG (1) or ab184147 (2). The precipitated DNA was detected by PCR with primer set targeting to Myc promoter (1 and 2).
Immunofluorescence analysis of 4% paraformaldehyde fixed U2OS cells, labeling MCM6 with ab184147 at a 1/500 dilution (left image), and costained with Hoescht 33342 (right image).
Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling MCM6 with ab184147 at a 1/500 dilution.
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling MCM6 with ab184147 at a 1/500 dilution.
All lanes : Anti-MCM6 antibody - ChIP Grade (ab184147) at 1/1000 dilutionLane 1 : 293T lysateLane 2 : A431 lysateLane 3 : HeLa lysateLane 4 : HepG2 lysateLysates/proteins at 30 µg per lane.