Anti-MCM7 antibody [47DC141]
| Name | Anti-MCM7 antibody [47DC141] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2360 |
| Prices | $404.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 47DC141 |
| Applications | IHC-P IP WB FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Dog, Human, Xenopus |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | MCM7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2360 - immunohistochemistry Formalin fixed paraffin embedded human tonsil stained with MCM7, using ABC and DAB chromagen.
This image shows immunostaining of rat brain endothelial cells. Brain endothelial cells were co-cultured with neuronal precursor cells and the nuclear staining represents cells in cell cycle. Primary antibody (ab2360) was used at 1:50 dilution, incubated overnight at 4 oC. Secondary antibody - Alexafluor (488 nm) at 1:200 dilution, incubated for 2 hours at room temperature.The picture was kindly supplied by Dr Joseph Corteza Lim and Dr Margery Barrand from University of Cambridge, Department of Pharmacology.
![All lanes : Anti-MCM7 antibody [47DC141] (ab2360) at 1/200 dilutionLane 1 : M phase Xenopus laevis egg extract, whole tissue lysate.Lane 2 : I phase Xenopus laevis egg extract, whole tissue lysate.SecondaryHRP conjugated Donkey anti-rabbit IgGdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/20580_ab2360_3a.jpg)
All lanes : Anti-MCM7 antibody [47DC141] (ab2360) at 1/200 dilutionLane 1 : M phase Xenopus laevis egg extract, whole tissue lysate.Lane 2 : I phase Xenopus laevis egg extract, whole tissue lysate.SecondaryHRP conjugated Donkey anti-rabbit IgGdeveloped using the ECL technique
ab2360 staining MCM7 in human bladder cancer tissue sections by Immunohistochemistry (formalin fixed sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 5% BSA for 1 hour at room temperature followed by incubation with the primary antibody at a 1/1200 dilution for 1 hour. A HRP-conjugated goat anti-mouse polyclonal was used as secondary antibody un-diluted.See Abreview
![Lane 1 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/50 dilutionLane 2 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/200 dilutionLane 3 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/500 dilutionLane 1 : Whole cell lysate prepared from SW780 cellsLane 2 : Whole cell lysate prepared from SW780 cellsLane 3 : Whole cell lysate prepared from SW780 cellsLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG conjugated to HRP at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/20582_MCM7-Primary-antibodies-ab2360-6.jpg)
Lane 1 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/50 dilutionLane 2 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/200 dilutionLane 3 : Anti-MCM7 antibody [47DC141] (ab2360) at 1/500 dilutionLane 1 : Whole cell lysate prepared from SW780 cellsLane 2 : Whole cell lysate prepared from SW780 cellsLane 3 : Whole cell lysate prepared from SW780 cellsLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG conjugated to HRP at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab2360 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLA cells stained with ab2360 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2360, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/20584_MCM7-Primary-antibodies-ab2360-8.jpg)
Overlay histogram showing HeLA cells stained with ab2360 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2360, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab2360 staining MCM7 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue tissue by Immunohistochemistry.
Product References
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