MCM7

Gene Symbol MCM7
Entrez Gene 4176
Alt Symbol CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104
Species Human
Gene Type protein-coding
Description minichromosome maintenance complex component 7
Other Description CDC47 homolog|DNA replication licensing factor MCM7|homolog of S. cerevisiae Cdc47|minichromosome maintenance deficient 7|protein phosphatase 1, regulatory subunit 104
Swissprots E9PGN9 A4D2A2 A4D2A1 Q15076 P33993 Q96GL1 Q96D34
Accessions CBX85483 CDH61696 EAL23855 EAL23856 EAW76598 EAW76599 EAW76600 P33993 AF279900 AAK07555 AI083651 AK055379 BAG51508 AK096959 BAG53399 AK226175 AK293172 BAG56716 AK314575 BAG37153 AY007130 AAG01994 BC009398 AAH09398 BC013375 AAH13375 D55716 BAA09534 D86748 BAH84845 DA253520 DA397394 DA538092 DQ895947 ABM86873 EU176445 ABW03896 X74796 CAA52803 XM_005250348 XP_005250405 NM_001278595 NP_001265524 NM_005916 NP_005907 NM_182776 NP_877577
Function Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. {ECO:0000269|PubMed:15210935, ECO:0000269|PubMed:15538388, ECO:0000269|PubMed:9305914}.
Subcellular Location Nucleus {ECO:0000250}.
Top Pathways DNA replication, Cell cycle

MCM7 (D10A11) XP ® Rabbit mAb - 3735 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10A11
Western blot analysis of extracts from various cell types using MCM7 (D10A11) XP ® Rabbit mAb.

MCM7 Antibody - 4018 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using MCM7 Antibody.

MCM7 (H-5) - sc-374403 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MCM7 (H-5): sc-374403. Western blot analysis of MCM7 expression in COLO 320DM whole cell lysate.

MCM7 (47DC141) - sc-56324 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MCM7 (47DC141): sc-56324. Western blot analysis of MCM7 expression in A-431 whole cell lysate.

MCM7 (47DC141) - sc-65475 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MCM7 (47DC141): sc-65475. Western blot analysis of MCM7 expression in non-transfected 293T: sc-117752 (A), human MCM7 transfected 293T: sc-173959 (B) and A-431 (C) whole cell lysates.

MCM7 (DCS-141) - sc-65469 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MCM7 (DCS-141): sc-65469. Western blot analysis of MCM7 expression in COLO 320DM (A), H4 (B) and HeLa (C) whole cell lysates.

MCM7 (G-7) - sc-46687 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MCM7 (G-7): sc-46687. Western blot analysis of MCM7 expression in COLO 320DM (A), H4 (B) and HeLa (C) whole cell lysates.

MCM7 (H-300) - sc-22782 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MCM7 (H-300): sc-22782. Western blot analysis of MCM7 expression in COLO 320DM (A), H4 (B) and HeLa (C) whole cell lysates and mouse thymus tissue extract (D).

MCM7 (141.2) - sc-9966 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MCM7 (141.2): sc-9966. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human lung tissue showing nuclear staining.

MCM7 (N-20) - sc-9847 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MCM7 (N-20): sc-9847. Western blot analysis of MCM7 expression in untreated (A) and PMA-treated (B) A-431 whole cell lysates.

MCM7 (SPM379) - sc-56429 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MCM7 (SPM379): sc-56429. Western blot analysis of MCM7 expression in COLO 320DM (A), H4 (B) and HeLa (C) whole cell lysates.

MCM7 (V-17) - sc-9794 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MCM7 (V-17): sc-9794. Western blot analysis of MCM7 expression in COLO 320DM (A), H4 (B) and HeLa (C) whole cell lysates and mouse thymus tissue extract (D).

MCM7 (0.N.194) - sc-71550 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MCM7 (0.N.194): sc-71550. Western blot analysis of MCM7 expression in COLO 320DM (A), H4 (B) and HeLa (C) whole cell lysates.

Anti-MCM7 antibody - ab51062 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MCM7 antibody (ab51062) at 1/5000 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-Rabbit HRP labeled. at 1/2000 dilution

Anti-MCM7 antibody - ab92385 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MCM7 antibody (ab92385) at 0.4 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-MCM7 antibody - ab3732 from Abcam

Prices $376.00
Sizes 50 µl
Host Goat
Clonality Polyclonal

Anti-MCM7 antibody [47DC141] - ab2360 from Abcam

Prices $404.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 47DC141
ab2360 - immunohistochemistry Formalin fixed paraffin embedded human tonsil stained with MCM7, using ABC and DAB chromagen.

Anti-MCM7 antibody [47DC141 or DCS-141] (Biotin) - ab79802 from Abcam

Prices $373.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone 47DC141 or DCS-141

Anti-MCM7 antibody [DCS-141] - ab49972 from Abcam

Host Mouse
Clonality Monoclonal
Clone DCS-141

Anti-MCM7 antibody [EP1974Y] - ab52489 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1974Y
MCM7 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit monoclonal to MCM7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52489.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 81kDa; MCM7

Anti-MCM7 antibody [EP1974Y] (Alexa Fluor® 488) - ab199753 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1974Y
ab199753 staining MCM7 in MCF7 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199753 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-MCM7 antibody [EPR1973Y] - ab134194 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1973Y
All lanes : Anti-MCM7 antibody [EPR1973Y] (ab134194) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-MCM7 antibody - C-terminal - ab188626 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling MCM7 with ab188626 at 1/50 dilution.

Anti-MCM7 Antibody (aa300-350) - LS-C287059 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Goat
Clonality Polyclonal
Detection of Human MCM7 by Western Blot. Sample: Whole cell lysate (60 ug) from HeLa cells. Antibody: Affinity purified goat anti-MCM7 used at 0.25 ug/ml. Detection: Chemiluminescence.

Anti-MCM7 Antibody (C-Terminus) IHC-plus™ - LS-B2009 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-MCM7 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2009 dilution 1:50.
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