Anti-MCM7 antibody [EP1974Y]

• Supplier: Abcam
• Catalog: ab52489
• Prices: $392.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1974Y
• Applications: ICC/IF ICC/IF WB IP ICC/IF FC IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues near the C terminus of human MCM7
• Description: Rabbit Monoclonal
• Gene: MCM7
• Conjugate: Unconjugated

Product images

MCM7 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit monoclonal to MCM7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52489.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 81kDa; MCM7

Anti-MCM7 antibody [EP1974Y] (ab52489) at 1/10000 dilution + Hela cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded human lung tissue using ab52489 at a 1/100 dilution.Immunohistochemical analysis of paraffin-embedded human lung tissue using ab52489 at a 1/100 dilution.

ICC/IF image of ab52489 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2489, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing HeLa cells stained with ab52489 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52489, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

Deregulation of the replisome factor MCMBP prompts oncogenesis in colorectal - Deregulation of the replisome factor MCMBP prompts oncogenesis in colorectal

Quimbaya M, Raspe E, Denecker G, De Craene B, Roelandt R, Declercq W, Sagaert X, De Veylder L, Berx G. Neoplasia. 2014 Sep;16(9):694-709.

Lethal synergism of 2009 pandemic H1N1 influenza virus and Streptococcus - Lethal synergism of 2009 pandemic H1N1 influenza virus and Streptococcus

Kash JC, Walters KA, Davis AS, Sandouk A, Schwartzman LM, Jagger BW, Chertow DS, Li Q, Kuestner RE, Ozinsky A, Taubenberger JK. MBio. 2011 Sep 20;2(5). pii: e00172-11.

The MCM-binding protein ETG1 aids sister chromatid cohesion required for - The MCM-binding protein ETG1 aids sister chromatid cohesion required for

Takahashi N, Quimbaya M, Schubert V, Lammens T, Vandepoele K, Schubert I, Matsui M, Inze D, Berx G, De Veylder L. PLoS Genet. 2010 Jan 15;6(1):e1000817.

MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA - MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA

Xu X, Rochette PJ, Feyissa EA, Su TV, Liu Y. EMBO J. 2009 Oct 7;28(19):3005-14.