Anti-MDA5 antibody
| Name | Anti-MDA5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab69983 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthesized peptide derived from C-terminal of human MDA5 |
| Description | Rabbit Polyclonal |
| Gene | IFIH1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MDA5 antibody (ab69983) at 1/500 dilutionLane 1 : Extracts from Jurkat cells (5-30µg) Lane 2 : Extracts from Jurkat cells (5-30µg) plus immunising peptide (5-10µg)
Ab69983 (2µg/ml) staining MDA5 in human thyroid gland using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and moderate nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab69983 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69983, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Functional effects of Toll-like receptor (TLR)3, 7, 9, RIG-I and MDA-5 - Functional effects of Toll-like receptor (TLR)3, 7, 9, RIG-I and MDA-5
Tengroth L, Millrud CR, Kvarnhammar AM, Kumlien Georen S, Latif L, Cardell LO. PLoS One. 2014 Jun 2;9(6):e98239.
Pattern recognition receptor-initiated innate antiviral response in mouse adipose - Pattern recognition receptor-initiated innate antiviral response in mouse adipose
Yu L, Yan K, Liu P, Li N, Liu Z, Zhu W, Chen Y, Han D. Immunol Cell Biol. 2014 Feb;92(2):105-15.
Stochastic expression of the interferon-beta gene. - Stochastic expression of the interferon-beta gene.
Zhao M, Zhang J, Phatnani H, Scheu S, Maniatis T. PLoS Biol. 2012 Jan;10(1):e1001249.
Human polynucleotide phosphorylase selectively and preferentially degrades - Human polynucleotide phosphorylase selectively and preferentially degrades
Das SK, Sokhi UK, Bhutia SK, Azab B, Su ZZ, Sarkar D, Fisher PB. Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11948-53. doi: