IFIH1

Gene Symbol IFIH1
Entrez Gene 64135
Alt Symbol AGS7, Hlcd, IDDM19, MDA-5, MDA5, RLR-2, SGMRT1
Species Human
Gene Type protein-coding
Description interferon induced with helicase C domain 1
Other Description CADM-140 autoantigen|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RIG-I-like receptor 2|RNA helicase-DEAD box protein 116|clinically amyopathic dermatomyositis autoantigen 140 kDa|helicard|helicase with 2 CARD domains|interferon-induced helicase C domain-containing protein 1|melanoma differentiation associated protein-5|melanoma differentiation-associated protein 5|murabutide down-regulated protein
Swissprots Q9H3G6 Q2NKL6 Q6DC96 Q86X56 Q96MX8 Q9BYX4
Accessions AAX93137 AAY24206 EAX11352 Q9BYX4 AF095844 AAG34368 AK056293 BAB71141 AK292941 BAF85630 AK314611 AY017378 AAG54076 BC007966 BC046208 AAH46208 BC078180 AAH78180 BC111750 AAI11751 BQ772836 BU902097 XM_011511628 XP_011509930 XM_011511629 XP_011509931 NM_022168 NP_071451
Function Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O- methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to
Subcellular Location Cytoplasm {ECO:0000269|PubMed:11805321, ECO:0000269|PubMed:14645903}. Nucleus {ECO:0000305}. Note=May be found in the nucleus, during apoptosis.
Tissue Specificity Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung. {ECO:0000269|PubMed:11805321, ECO:0000269|PubMed:12015121, ECO:0000269|PubMed:14645903}.
Top Pathways RIG-I-like receptor signaling pathway, Measles, Influenza A, Herpes simplex infection

MDA-5 (D74E4) Rabbit mAb - 5321 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D74E4
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human MDA-5 (+), using MDA-5 (D74E4) Rabbit mAb.

MDA-5 (K375) Antibody - 4109 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from differentiated THP-1 cells, untreated (-) or treated with LPS (1 μg/ml, overnight), using MDA-5 (K375) Antibody.

MDA-5 (R470) Antibody - 4110 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

MDA5 (C-5) - sc-365630 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MDA5 (C-5): sc-365630. Western blot analysis of MDA5 expression in Jurkat whole cell lysate.

MDA5 (H-61) - sc-134513 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MDA5 (H-61): sc-134513. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

MDA5 (C-16) - sc-48031 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MDA5 (C-16): sc-48031. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.

Anti-MDA5 antibody - ab155162 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MDA5 antibody (ab155162) at 1/1000 dilution + Raji whole cell lysate at 30 µg

Anti-MDA5 antibody - ab172733 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-MDA5 antibody (ab172733) at 1 µg/mlLane 1 : MDA5-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-MDA5 antibody [EPR6743] - ab126630 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6743
Anti-MDA5 antibody [EPR6743] (ab126630) at 1/1000 dilution + THP1 treated with LPS cell lysate at 10 µgSecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Anti-MDA5 antibody - N-terminal - ab187303 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MDA5 antibody - N-terminal (ab187303) at 1/1000 dilutionLane 1 : mouse thymus lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysate

Anti-MDA5 antibody - ab69983 from Abcam

Prices $391.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MDA5 antibody (ab69983) at 1/500 dilutionLane 1 : Extracts from Jurkat cells (5-30µg) Lane 2 : Extracts from Jurkat cells (5-30µg) plus immunising peptide (5-10µg)

Anti-MDA5 antibody - ab79055 from Abcam

Prices $379.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed human pancreas using 5 µg/ml ab79055

Anti-MDA5 antibody - ab4544 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Ab4544 (10ug/ml) staining human MDA5 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Ab4544 shows strong cytoplasmic staining of some cells in the islets of Langerhans.

Anti-MDA5 antibody - ab118377 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ab118377, at 3ug/ml, staining MDA5 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry.

Anti-IFIH1 / MDA5 Antibody (C-Terminus) IHC-plus™ - LS-B2107 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MDA5 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2107 concentration 5 ug/ml.

Anti-IFIH1 / MDA5 Antibody (aa612-661) IHC-plus™ - LS-B5622 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MDA5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5622 concentration 5 ug/ml.

Anti-IFIH1 / MDA5 Antibody (Internal) IHC-plus™ - LS-B2349 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MDA5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2349 concentration 5 ug/ml.

Anti-IFIH1 / MDA5 Antibody (aa464-476) - LS-C55098 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-IFIH1 / MDA5 Antibody (aa2-14) - LS-C55097 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (10 ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Anti-IFIH1 / MDA5 Antibody (Arg470) - LS-C191022 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal

Anti-IFIH1 / MDA5 Antibody (aa145-160) - LS-C194010 from LifeSpan Bioscience

Prices $905.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-IFIH1 / MDA5 Antibody - LS-C308389 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-IFIH1 / MDA5 Antibody - LS-C310700 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-IFIH1 / MDA5 Antibody - LS-C310701 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-IFIH1 / MDA5 Antibody (Internal, clone EPR6743) - LS-C152270 from LifeSpan Bioscience

Prices $480.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6743
Western blot analysis on THP-1 treated with LPS cell lysate using anti-MDA5 antibody.
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