Anti-MDA5 antibody
| Name | Anti-MDA5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab79055 |
| Prices | $379.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | 16 amino acid peptide from near the carboxy terminus of human MDA5 |
| Description | Rabbit Polyclonal |
| Gene | IFIH1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed human pancreas using 5 µg/ml ab79055
ICC/IF image of ab79055 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79055, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Astrogliosis involves activation of retinoic acid-inducible gene-like signaling - Astrogliosis involves activation of retinoic acid-inducible gene-like signaling
de Rivero Vaccari JP, Minkiewicz J, Wang X, De Rivero Vaccari JC, German R, Marcillo AE, Dietrich WD, Keane RW. Glia. 2012 Mar;60(3):414-21.
Neutrophils express distinct RNA receptors in a non-canonical way. - Neutrophils express distinct RNA receptors in a non-canonical way.
Berger M, Hsieh CY, Bakele M, Marcos V, Rieber N, Kormann M, Mays L, Hofer L, Neth O, Vitkov L, Krautgartner WD, von Schweinitz D, Kappler R, Hector A, Weber A, Hartl D. J Biol Chem. 2012 Jun 1;287(23):19409-17.