Anti-MDMX antibody

• Supplier: Abcam
• Catalog: ab16058
• Prices: $378.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human MDMX Read Abcam's proprietary immunogen policy (Peptide available as ab16383
• Description: Rabbit Polyclonal
• Gene: MDM4
• Conjugate: Unconjugated

Product images

All lanes : Anti-MDMX antibody (ab16058) at 1/1000 dilutionLane 1 : 20ug calf thymus histoneLane 2 : 20ug HeLa nuclear extractLane 3 : 20ug calf thymus histone with Human MDMX peptide (ab16383) at 1 µg/mlLane 4 : 20ug HeLa nuclear extract with Human MDMX peptide (ab16383) at 1 µg/mlSecondaryAlexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilutionPerformed under reducing conditions.

ICC/IF image of ab16058 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16058, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

Product References

A Fluorescent Probe for Imaging p53-MDM2 Protein-Protein Interaction. - A Fluorescent Probe for Imaging p53-MDM2 Protein-Protein Interaction.

Liu Z, Miao Z, Li J, Fang K, Zhuang C, Du L, Sheng C, Li M. Chem Biol Drug Des. 2015 Apr;85(4):411-7.

MDM2 antagonist nutlin-3 displays antiproliferative and proapoptotic activity in - MDM2 antagonist nutlin-3 displays antiproliferative and proapoptotic activity in

Tabe Y, Sebasigari D, Jin L, Rudelius M, Davies-Hill T, Miyake K, Miida T, Pittaluga S, Raffeld M. Clin Cancer Res. 2009 Feb 1;15(3):933-42.