MDM4

Gene Symbol MDM4
Entrez Gene 4194
Alt Symbol HDMX, MDMX, MRP1
Species Human
Gene Type protein-coding
Description MDM4, p53 regulator
Other Description MDM4 protein variant G|MDM4 protein variant Y|MDM4-related protein 1|Mdm4 p53 binding protein homolog|double minute 4, human homolog of; p53-binding protein|mdm2-like p53-binding protein|protein Mdm4|protein Mdmx
Swissprots Q8IV83 O15151 Q32SL2 Q2M2Y2 Q6GS18
Accessions AAO13494 EAW91515 EAW91516 O15151 AB209383 BAD92620 AF007111 AAB62928 AK097188 AK130328 AK223228 BAD96948 AK303626 BAG64634 AK304698 BAG65468 AK310607 AK310903 AY923176 AAY22054 AY949028 AAX51227 BC025993 BC067299 AAH67299 BC105106 AAI05107 BC143431 BC143432 BM475824 BP247507 BP334671 BX108199 BX640923 CAE45961 CA395099 CR749470 CAH18300 KC479043 AGJ70143 KF435149 AGZ93683 KF435150 AGZ93684 XM_006711328 XP_006711391 XM_011509565 XP_011507867 XM_011509566 XP_011507868 XM_011509567 XP_011507869 XM_011509568 XP_011507870 NM_001204171 NP_001191100 NM_001204172 NP_001191101 NM_001278516 NP_001265445 NM_001278517 NP_001265446 NM_001278518 NP_001265447 NM_001278519 NP_001265448 NM_002393 NP_002384
Function Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. {ECO:0000269|PubMed:16163388, ECO:0000269|PubMed:16511572}.
Subcellular Location Nucleus.
Tissue Specificity Expressed in all tissues tested with high levels in thymus.
Top Pathways p53 signaling pathway, MicroRNAs in cancer

MDMX (D-4) - sc-74468 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MDMX (D-4): sc-74468. Western blot analysis of MDMX expression in Sol8 whole cell lysate.

MDMX (D-2) - sc-365902 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MDMX (D-2): sc-365902. Western blot analysis of MDMX expression in non-transfected: sc-117752 (A) and human MDMX transfected: sc-111488 (B) 293T whole cell lysates.

MDMX (G-10) - sc-74467 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MDMX (G-10): sc-74467. Western blot analysis of MDMX expression in in Sol8 whole cell lysate (A) and Hep G2 nuclear extract (B).

MDMX (A-11) - sc-374147 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MDMX (A-11): sc-374147. Western blot analysis of MDMX expression in non-transfected: sc-117752 (A) and human MDMX transfected: sc-111488 (B) 293T whole cell lysates.

MDMX (H-130) - sc-28222 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MDMX (H-130): sc-28222. Western blot analysis of MDMX expression in U-2 OS whole cell lysate.

MDMX (D-19) - sc-14738 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MDMX (D-19): sc-14738. Western blot analysis of MDMX expression in MCF7 (A), HeLa (B) and U-2 OS (C) whole cell lysates and HeLa nuclear extract (D).

MDMX (C-18) - sc-14740 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MDMX (C-18): sc-14740. Western blot analysis of MDMX expression in non-transfected: sc-117752 (A) and human MDMX transfected: sc-111488 (B) 293T whole cell lysates.

Anti-MDMX (phospho S367) antibody [15] - ab122926 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 15
All lanes : Anti-MDMX (phospho S367) antibody [15] (ab122926) at 1 µg/mlLane 1 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM)Lane 2 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 1 hourLane 3 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 2 hourLane 4 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 3 hourLane 5 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 4 hourLane 6 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 1 hourLane 7 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 2 hourLane 8 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 3 hourLane 9 : MCF cells preincubated with the

Anti-MDMX (phospho S403) antibody - ab70236 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MDMX (phospho S403) antibody (ab70236) at 0.13 µg/mlLane 1 : 293T whole cell lysate with HA tagged-MDMX S403A mutant and NCS (2hrs at 200 ng/ml)Lane 2 : 293T whole cell lysate with HA tagged-MDMX S403A mutant and NCS (0.5hrs at 200 ng/ml)Lane 3 : 293T whole cell lysate with HA tagged-MDMX S403A mutantLane 4 : 293T whole cell lysate with HA tagged-MDMX (wild-type) and NCS (2hrs at 200 ng/ml)Lane 5 : 293T whole cell lysate with HA tagged-MDMX(wild-type) and NCS (0.5hrs at 200 ng/ml)Lane 6 : 293T whole cell lysate with HA tagged-MDMX (wild-type)Lane 7 : mock transfected 293T whole cell lysateLysates/proteins at 30 µg per lane.developed using the ECL techniqueObserved band size : 80 kDa (why is the actual band size different from the predicted?)Exposure time : 30 seconds

Anti-MDMX (phospho Y55) antibody [PH-MDMX-55] - ab62726 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone PH-MDMX-55

Anti-MDMX (phospho Y99) antibody [PH-MDMX-169] - ab62728 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone PH-MDMX-169

Anti-MDMX antibody - ab16058 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MDMX antibody (ab16058) at 1/1000 dilutionLane 1 : 20ug calf thymus histoneLane 2 : 20ug HeLa nuclear extractLane 3 : 20ug calf thymus histone with Human MDMX peptide (ab16383) at 1 µg/mlLane 4 : 20ug HeLa nuclear extract with Human MDMX peptide (ab16383) at 1 µg/mlSecondaryAlexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilutionPerformed under reducing conditions.

Anti-MDMX antibody - ab154324 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MDMX antibody (ab154324) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

Anti-MDMX antibody - ab190364 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation of Jurkat cell lysates (1 mg for IP, 20% of IP loaded) using ab190364 at 6 µg/mg lysate (Lanes 1,2) or control IgG (Lane 3). Protein was detected using ab190364 at 1 µg/ml for western blotting.

Anti-MDMX antibody - ab167384 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-MDMX antibody (ab167384) at 1 µg/mlLane 1 : MDMX transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

Anti-MDMX antibody - ab84393 from Abcam

Host Rabbit
Clonality Polyclonal
ab84393, at 1/250 dilution, staining MDMX in formalin-fixed, paraffin-embedded human ovarian carcinoma by Immunohistochemistry, using DAB staining and a Goat anti-Rabbit IgG HRP conjugated secondary antibody at a dilution of 1/100.

Anti-MDMX antibody - ab95026 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MDMX antibody - ab157091 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab157091 at 1 µg/ml detecting MDMX in Jurkat whole cell lysate by WB following IP. Lane 1: ab157091 at 6 µg/mg of lysate Lane 2: IP with an antibody which recognizes MDMX Lane 3: Control IgG. In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-MDMX antibody [2D10F4] - ab76362 from Abcam

Host Mouse
Clonality Monoclonal
Clone 2D10F4
Immunohistochemical staining of paraffin-embedded Human cerebra tissue (left hand image) and human lung carcinoma tissue (right hand image) using ab76362 at 1/200 dilution with DAB staining.

Anti-MDMX antibody [MDMX-82] - ab49993 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone MDMX-82
Anti-MDMX antibody [MDMX-82] (ab49993) at 1/1000 dilution + Mouse myoblast cell line C2C12 whole cell lysate at 15 µgSecondaryHRP-conjugated sheep anti-mouse IgG polyclonal at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MDM4 / MDMX Antibody - LS-C48685 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MDM4 / MDMX Antibody (C-Terminus) - LS-C46831 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MDM4 / MDMX Antibody (aa125-175) - LS-C67243 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MDM4 / MDMX Antibody (aa101-150) - LS-C31169 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
MDM4 / MDMX antibody LS-C31169 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.

Anti-MDM4 / MDMX Antibody (aa128-157) - LS-C164109 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MDM4 Antibody western blot of ZR-75-1 cell line lysates (35 ug/lane). The MDM4 antibody detected the MDM4 protein (arrow).
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