Anti-MEF2A (phospho T312) antibody
| Name | Anti-MEF2A (phospho T312) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab30644 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ELISA WB IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic phosphopeptide derived from human MEF2A around the phosphorylation site of Threonine 312 |
| Description | Rabbit Polyclonal |
| Gene | MEF2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-MEF2A (phospho T312) antibody (ab30644) at 1/1000 dilution + Mouse liver tissue lysate - whole at 40 µgSecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: using ab30644 (10ug/ml); right: the same antibody preincubated with synthesized phosphopeptide.
All lanes : Anti-MEF2A (phospho T312) antibody (ab30644) at 1 µg/mlLane 1 : Untreated NIH/3T3 cells (5-30ug).Lane 2 : NIH/3T3 cells treated with PMA (5-30ug)
ICC/IF image of ab30644 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30644, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Flow activation of AMP-activated protein kinase in vascular endothelium leads to - Flow activation of AMP-activated protein kinase in vascular endothelium leads to
Young A, Wu W, Sun W, Benjamin Larman H, Wang N, Li YS, Shyy JY, Chien S, Garcia-Cardena G. Arterioscler Thromb Vasc Biol. 2009 Nov;29(11):1902-8. doi:
A retrograde neuronal survival response: target-derived neurotrophins regulate - A retrograde neuronal survival response: target-derived neurotrophins regulate
Pazyra-Murphy MF, Hans A, Courchesne SL, Karch C, Cosker KE, Heerssen HM, Watson FL, Kim T, Greenberg ME, Segal RA. J Neurosci. 2009 May 20;29(20):6700-9.