Anti-MEF2D antibody
| Name | Anti-MEF2D antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab32845 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP EMSA IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 292-514 of Human MEF2D |
| Description | Rabbit Polyclonal |
| Gene | MEF2D |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunoprecipitation of 35S-methionine labelled HeLa cells with anti-MEF2D sera. 1: pre-immune sera; 2: ab32845 anti-MEF2D sera.
ab32845 (2µg/ml) staining MEF2D in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers - The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers
Pearen MA, Eriksson NA, Fitzsimmons RL, Goode JM, Martel N, Andrikopoulos S, Muscat GE. Mol Endocrinol. 2012 Mar;26(3):372-84.
alpha-synuclein aggregation reduces nigral myocyte enhancer factor-2D in - alpha-synuclein aggregation reduces nigral myocyte enhancer factor-2D in
Chu Y, Mickiewicz AL, Kordower JH. Neurobiol Dis. 2011 Jan;41(1):71-82.
Signal-dependent activation of the MEF2 transcription factor by dissociation from - Signal-dependent activation of the MEF2 transcription factor by dissociation from
Lu J, McKinsey TA, Nicol RL, Olson EN. Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4070-5.
Ca(2+)-dependent gene expression mediated by MEF2 transcription factors. - Ca(2+)-dependent gene expression mediated by MEF2 transcription factors.
Blaeser F, Ho N, Prywes R, Chatila TA. J Biol Chem. 2000 Jan 7;275(1):197-209.
Regulatory role of MEF2D in serum induction of the c-jun promoter. - Regulatory role of MEF2D in serum induction of the c-jun promoter.
Han TH, Prywes R. Mol Cell Biol. 1995 Jun;15(6):2907-15.