Ab28834 staining MEK2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Left image: untreated. Right image: preincubated with synthesized peptide.
All lanes : Anti-MEK2 antibody (ab28834) at 1/500 dilution (extracts from ovary canner cells (5-30ug).)Lane 1 : Line 1: using MEK2 antibodyLane 2 : Line 2: the same antibody preincubated with synthesized peptide.
ICC/IF image of ab28834 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28834, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.