Anti-MEK3 + MEK6 antibody [EPR17340]

• Supplier: Abcam
• Catalog: ab200831
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17340
• Applications: IHC-P WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MEK3 + MEK6 aa 300 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: MAP2K3
• Conjugate: Unconjugated

Product images

Anti-MEK3 + MEK6 antibody [EPR17340] (ab200831) at 1/10000 dilution + Human MEK6 recombinant protein fragment at 10 µg

All lanes : Anti-MEK3 + MEK6 antibody [EPR17340] (ab200831) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-MEK3 + MEK6 antibody [EPR17340] (ab200831) at 1/10000 dilution + Human fetal liver lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-MEK3 + MEK6 antibody [EPR17340] (ab200831) at 1/1000 dilutionLane 1 : Mouse spleen lysate at 10 µgLane 2 : Rat spleen lysate at 10 µgLane 3 : C6 (Rat glial tumor cells) whole cell lysateLane 4 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 6 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling MEK3 + MEK6 with ab200831 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling MEK3 + MEK6 with ab200831 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on Human spleen tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MEK3 + MEK6 with ab200831 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on Mouse liver tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

MEK3 + MEK6 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200831 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab200831 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab200831 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200831 in Jurkat whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 seconds.