MAP2K3

Gene Symbol MAP2K3
Entrez Gene 5606
Alt Symbol MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase kinase 3
Other Description MAP kinase kinase 3|MAPK/ERK kinase 3|MAPKK 3|MEK 3|SAPK kinase 2|dual specificity mitogen-activated protein kinase kinase 3|stress-activated protein kinase kinase 2
Swissprots B3KSK7 P46734 Q9UE72 Q9UE71 Q99441
Accessions AAB40653 EAW50386 P46734 AB451226 BAG70040 AB451349 BAG70163 AI758302 AK093838 BAG52769 AK223490 BAD97210 AK315505 BAG37889 BC032478 AAH32478 BM836867 CR536514 CAG38752 CR541793 CAG46592 D87116 BAA13248 DA131175 DA987047 DQ894188 ABM85114 GQ225578 ACT33197 L36719 AAC41718 U66839 AAB40652 XM_005256722 XP_005256779 XM_005256723 XP_005256780 XM_011523958 XP_011522260 XM_011523959 XP_011522261 NM_002756 NP_002747 NM_145109 NP_659731 NM_145110
Function Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Tissue Specificity Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Top Pathways Rap1 signaling pathway, Toll-like receptor signaling pathway, Inflammatory mediator regulation of TRP channels, TNF signaling pathway, Epstein-Barr virus infection

Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb - 9236 from Cell Signaling Technology

Prices $307.00, $719.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 22A8
Western blot analysis of extracts from untreated or UV-treated COS, NIH/3T3 and C6 cells, using Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb.

Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody - 9231 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated or anisomycin-treated for the indicated times, using Phospho-MKK3/MKK6 (Ser189/207) Antibody (upper) or MKK3 Antibody #9232 (lower).

Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb - 12280 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8E9
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb (upper), MKK3 (D4C3) Rabbit mAb #8535 (middle), or MKK6 (D31D1) Rabbit mAb #8550 (lower).

MKK3 (D4C3) Rabbit mAb - 8535 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4C3
Western blot analysis of extracts from various cell lines using MKK3 (D4C3) Rabbit mAb.

MKK3b Antibody - 9238 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from C6, NIH/3T3 and 293 cells, using MKK3b Antibody #9238.

MKK3 Antibody - 5674 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using MKK3 Antibody.

PhosphoPlus® MKK3/MKK6 (Ser189/207) Antibody Kit - 9230 from Cell Signaling Technology

Prices $489.00
Sizes 1 kit (10 western blots)
Clonality Polyclonal
Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.

MKK3 Antibody - 9232 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

MEK-3 (C-19) - sc-961 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MEK-3 (C-19): sc-961. Western blot analysis of MEK-3 expression in Jurkat (A) and K-562 (B) whole cell lysates.

MEK-3 (N-20) - sc-959 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MEK-3 (N-20): sc-959. Western blot analysis of MEK-3 expression in HeLa (A), Jurkat (B), NIH/3T3 (C), WEHI-231 (D) and CTLL-2 (E) whole cell lysates.

MEK-3 (I-20) - sc-960 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MEK-3 (I-20): sc-960. Western blot analysis of MEK-3 expression in NIH/3T3 (A) and Src-3T3 (B) whole cell lysates.

MEK-3 (B-2) - sc-376627 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MEK-3 (B-2): sc-376627. Western blot analysis of MEK-3 expression in non-transfected: sc-117752 (A) and human MEK-3 transfected: sc-114954 (B) 293T whole cell lysates.

MEK-3 (B-5) - sc-271779 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MEK-3 (B-5): sc-271779. Western blot analysis of MEK-3 expression in non-transfected: sc-110760 (A) and human MEK-3 transfected: sc-158720 (B) 293 whole cell lysates.

MEK-3 (30) - sc-135985 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
MEK-3 (30): sc-135985. Western blot analysis of MEK-3 expression in HeLa whole cell lysate.

MEK-3 (5F7) - sc-81474 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
MEK-3 (5F7): sc-81474. Western blot analysis of MEK-3 expression in non-transfected: sc-117752 (A) and human MEK-3 transfected: sc-114954 (B) 293T whole cell lysates.

MEK-3/6 (H-90) - sc-13069 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MEK-3/6 (H-90): sc-13069. Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.

MEK-3/6 (D-3) - sc-133230 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MEK-3/6 (D-3): sc-133230. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells.

MEK-3/6 (B-1) - sc-136982 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MEK-3/6 (B-1): sc-136982. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

MEK-3/6 (E-3) - sc-137106 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MEK-3/6 (E-3): sc-137106. Western blot analysis of MEK-3 expression in non-transfected: sc-117752 (A) and human MEK-3 transfected: sc-114954 (B) 293T whole cell lysates.

p-MEK-3 (Ser 189) - sc-293107 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-MEK-3 (Ser 189): sc-293107. Western blot analysis of MEK-3 phosphorylation expression in untreated (A) and PMA treated (B) HeLa whole cell lysates.

p-MEK-3/6 (B-9) - sc-8407 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of MEK-6 phosphorylation in untreated (A,C) and PMA induced (B,D) HeLa cells. Antibodies tested include p-MEK-3/6 (B-9): sc-8407 (A,B) and MEK-3 (C-19): sc-961 (C,D).

Anti-MEK3 + MEK6 (phospho S189) antibody - ab4759 from Abcam

Prices $412.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MEK3 + MEK6 antibody [EPR17340] - ab200831 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17340
Anti-MEK3 + MEK6 antibody [EPR17340] (ab200831) at 1/10000 dilution + Human MEK6 recombinant protein fragment at 10 µg

Anti-MEK3 + MEK6 antibody [EPR17345] - ab181555 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17345
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling MEK3 + MEK6 with ab181555 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm and nuclear staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-1. ab181555 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Anti-MEK3 (phospho S189) antibody - ab131283 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MEK3 (phospho S189) antibody (ab131283) at 1/500 dilutionLane 1 : HeLa cell extract untreated Lane 2 : HeLa cell extract treated with PMA
Search more