Anti-MEK5 (phospho S311 + T315) antibody
| Name | Anti-MEK5 (phospho S311 + T315) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70608 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB IP ELISA DB |
| Species Reactivities | Mouse, Rat, Chicken, Human, Dog |
| Antigen | Synthetic phospho-peptide (Human) surrounding the epitope SIAKT with double phosphorylation at sites Ser311 and Thr315 |
| Description | Rabbit Polyclonal |
| Gene | MAP2K5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MEK5 (phospho S311 + T315) antibody (ab70608) at 1/1000 dilutionLane 1 : Whole cell lysates derived from EGF-stimulated A431Lane 2 : Whole cell lysates derived from EGF-stimulated A431 with immunising peptide
1 µg peptide was spotted onto NC membrane and blotted using ab70608 at 1/1000 dilution. A:MEK5 (pS311/pT315), B: MEK5 (Non-phosphorylated), C: Non-related phosphospecific PP.
Immunohistochemical analysis of Human colorectal cancer tissue, staining MEK5 (phospho S311 + T315) with ab70608 at 1/800 dilution. Antigen retrieval was performed by heat mediation with citrate buffer (pH 6.0). Sections were incubated overnight at 4°C with the primary antibody and then incubated with the secondary antibody for 30 min at room temperature. Staining was detected using DAB.