Anti-MEKK2 antibody [EP626Y]
| Name | Anti-MEKK2 antibody [EP626Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab33918 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP626Y |
| Applications | IHC-F WB ICC/IF IHC-P IP FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues near the N terminus of MEKK2 (Human) |
| Description | Rabbit Monoclonal |
| Gene | MAP3K2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-MEKK2 antibody [EP626Y] (ab33918) at 1/50000 dilution + Jurkat cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/21598_33918.jpg)
Anti-MEKK2 antibody [EP626Y] (ab33918) at 1/50000 dilution + Jurkat cell lysate
![MEKK2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 10µg of Rabbit monoclonal [EP626Y] to MEKK2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33918.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .Band: 75kDa: MEKK2.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/21599_MEKK2-Primary-antibodies-ab33918-4.jpg)
MEKK2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 10µg of Rabbit monoclonal [EP626Y] to MEKK2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33918.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .Band: 75kDa: MEKK2.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab33918 at 1/50.
Overlay histogram showing HepG2 cells stained with ab33918 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33918, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
MAP kinase kinase kinase-2 (MEKK2) regulates hypertrophic remodeling of the right - MAP kinase kinase kinase-2 (MEKK2) regulates hypertrophic remodeling of the right
Brown RD, Ambler SK, Li M, Sullivan TM, Henry LN, Crossno JT Jr, Long CS, Garrington TP, Stenmark KR. Am J Physiol Heart Circ Physiol. 2013 Jan 15;304(2):H269-81. doi:
Overexpression of MEKK2 is associated with colorectal carcinogenesis. - Overexpression of MEKK2 is associated with colorectal carcinogenesis.
Jiang L, Huang M, Wang L, Fan X, Wang P, Wang D, Fu X, Wang J. Oncol Lett. 2013 Nov;6(5):1333-1337. Epub 2013 Aug 29.
Imaging mass spectrometry of a specific fragment of mitogen-activated protein - Imaging mass spectrometry of a specific fragment of mitogen-activated protein
Cazares LH, Troyer D, Mendrinos S, Lance RA, Nyalwidhe JO, Beydoun HA, Clements MA, Drake RR, Semmes OJ. Clin Cancer Res. 2009 Sep 1;15(17):5541-51.
Identification of a novel amino acid response pathway triggering ATF2 - Identification of a novel amino acid response pathway triggering ATF2
Chaveroux C, Jousse C, Cherasse Y, Maurin AC, Parry L, Carraro V, Derijard B, Bruhat A, Fafournoux P. Mol Cell Biol. 2009 Dec;29(24):6515-26.