Anti-MEKK3 antibody [ep599y]
| Name | Anti-MEKK3 antibody [ep599y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab40750 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | ep599y |
| Applications | WB IHC-P ICC/IF ICC/IF FC IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues in the N-terminal (Human) |
| Description | Rabbit Monoclonal |
| Gene | MAP3K3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HeLa cells stained with ab40750 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40750, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-MEKK3 antibody [ep599y] (ab40750) at 1/1000 dilution + Hela cell lysate at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/21610_40750_1.jpg)
Anti-MEKK3 antibody [ep599y] (ab40750) at 1/1000 dilution + Hela cell lysate at 10 µg
Immunohistochemical analysis of paraffin embedded human breast adenocarcinoma using ab40750 diluted 1:50.Immunohistochemical analysis of paraffin embedded human breast adenocarcinoma using ab40750 diluted 1:50.
Immunofluorescent staining of HeLa cells using ab40750 diluted 1:50.
Product References
Identification of a novel amino acid response pathway triggering ATF2 - Identification of a novel amino acid response pathway triggering ATF2
Chaveroux C, Jousse C, Cherasse Y, Maurin AC, Parry L, Carraro V, Derijard B, Bruhat A, Fafournoux P. Mol Cell Biol. 2009 Dec;29(24):6515-26.
Phosphorylation of MEKK3 at threonine 294 promotes 14-3-3 association to inhibit - Phosphorylation of MEKK3 at threonine 294 promotes 14-3-3 association to inhibit
Matitau AE, Scheid MP. J Biol Chem. 2008 May 9;283(19):13261-8.