Anti-MelanA antibody [A103]
| Name | Anti-MelanA antibody [A103] |
|---|---|
| Supplier | Abcam |
| Catalog | ab785 |
| Prices | $382.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | A103 |
| Applications | IHC-P IHC-F FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein (Mouse) |
| Description | Mouse Monoclonal |
| Gene | MLANA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab785 staining in Human Skin Melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab785, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing Malme-3 cells stained with ab785 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab785, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/21637_MelanA-Primary-antibodies-ab785-2.jpg)
Overlay histogram showing Malme-3 cells stained with ab785 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab785, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
The origin and role of autophagy in the formation of cytoplasmic granules in - The origin and role of autophagy in the formation of cytoplasmic granules in
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Human CD271-positive melanoma stem cells associated with metastasis establish - Human CD271-positive melanoma stem cells associated with metastasis establish
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Serological analysis of Melan-A(MART-1), a melanocyte-specific protein - Serological analysis of Melan-A(MART-1), a melanocyte-specific protein
Chen YT, Stockert E, Jungbluth A, Tsang S, Coplan KA, Scanlan MJ, Old LJ. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5915-9.