MLANA

Gene Symbol MLANA
Entrez Gene 2315
Alt Symbol MART-1, MART1
Species Human
Gene Type protein-coding
Description melan-A
Other Description antigen LB39-AA|antigen SK29-AA|melanoma antigen recognized by T-cells 1|protein Melan-A
Swissprots Q6ICU4 Q16655
Accessions CAC37866 CAD79827 EAW58755 EAW58756 Q16655 AK312149 BAG35083 BC014423 AAH14423 CR450299 CAG29295 DQ892257 ABM83183 DQ895459 ABM86385 U06452 AAA19238 U06654 AAA20389 NM_005511 NP_005502
Function Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. {ECO:0000269|PubMed:15695812, ECO:0000269|PubMed:19717472}.
Subcellular Location Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
Tissue Specificity Expression is restricted to melanoma and melanocyte cell lines and retina.

Melan-A (A103) - sc-20032 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Melan-A (A103) PE: sc-20032 PE. Intracellular FCM analysis of fixed and permeabilized B16-FO cells. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-2866.

Melan-A (D-1) - sc-271432 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Melan-A (D-1): sc-271432. Western blot analysis of Melan-A expression in SK-MEL-28 whole cell lysate.

Melan-A (B-10) - sc-271802 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Melan-A (B-10): sc-271802. Western blot analysis of Melan-A expression in SK-MEL-28 whole cell lysate.

Melan-A (C-20) - sc-8106 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Melan-A (C-20): sc-8106. Western blot analysis of Melan-A expression in B16-F0 whole cell lysate.

Melan-A (D-6) - sc-376642 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Melan-A (D-6): sc-376642. Western blot analysis of Melan-A expression in SK-MEL-28 whole cell lysate.

Melan-A (N-19) - sc-8107 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Melan-A (N-19): sc-8107. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skin tissue showing cytoplasmic staining.

Melan-A (FL-118) - sc-28871 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Melan-A (FL-118): sc-28871. Western blot analysis of Melan-A expression in non-transfected 293T: sc-117752 (A), human Melan-A transfected 293T: sc-159493 (B) and SK-MEL-28 (C) whole cell lysates.

Melan-A (M2-7C10) - sc-53536 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Melan-A (M2-7C10): sc-53536. Western blot analysis of Melan-A expression in SK-MEL-28 whole cell lysate.

Melan-A (M2-9E3) - sc-53537 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Melan-A (M2-9E3): sc-53537. Western blot analysis of Melan-A expression in SK-MEL-28 whole cell lysate.

Melan-A (3H2639) - sc-71566 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Melan-A (3H2639): sc-71566. Western blot analysis of Melan-A expression in non-transfected 293T: sc-117752 (A), human Melan-A transfected 293T: sc-159493 (B) and SK-MEL-28 (C) whole cell lysates.

Melan-A (0.N.397) - sc-71569 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Melan-A (0.N.397): sc-71569. Western blot analysis of Melan-A expression in SK-MEL-28 whole cell lysate.

Melan-A (SPM342) - sc-56431 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Melan-A (SPM342): sc-56431. Western blot analysis of Melan-A expression in C32 whole cell lysate.

Melan-A (0.N.396) - sc-71567 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Melan-A (0.N.396): sc-71567. Western blot analysis of Melan-A expression in non-transfected 293T: sc-117752 (A), human Melan-A transfected 293T: sc-159493 (B) and SK-MEL-28 (C) whole cell lysates.

Anti-MelanA antibody - ab15468 from Abcam

Host Rabbit
Clonality Polyclonal
ICC/IF image of ab15468 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15468 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-MelanA antibody - ab53135 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MelanA antibody (ab53135)Lane 1 : NIH/3T3 cellsLane 2 : NIH/3T3 cells with blocking peptide

Anti-MelanA antibody - ab199935 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MelanA antibody (ab199935) at 1/1000 dilution + Mouse thymus total cell extract

Anti-MelanA antibody - ab118440 from Abcam

Prices $376.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
ab118440 at 10ug/ml staining MelanA in Human melanoma (4 microns) tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-MelanA antibody - ab110559 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-MelanA antibody (ab110559) at 1/100 dilution + HL-60 cell line lysate at 35 µg

Anti-MelanA antibody [3E2] - ab140503 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E2
Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY MelanA, labelling MelanA with ab140503 at 1/100 dilution.

Anti-MelanA antibody [A103] - ab785 from Abcam

Prices $382.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone A103
IHC image of ab785 staining in Human Skin Melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab785, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MelanA antibody [A103], prediluted - ab51936 from Abcam

Prices $370.00
Sizes 5 ml
Host Mouse
Clonality Monoclonal
Clone A103

Anti-MelanA antibody [A103-M27C10-M29E3] - ab200544 from Abcam

Prices $359.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone A103-M27C10-M29E3
Immunohistochemical analysis of formalin fixed paraffin embedded human melanoma tissue labeling MelanA with ab200544 at 1 μg/mL.

Anti-MelanA antibody [Cocktail - HMB45+A103+T311], prediluted - ab82762 from Abcam

Prices $372.00
Sizes 6 ml
Host Mouse
Clonality Monoclonal
Clone Cocktail - HMB45+A103+T311

Anti-MelanA antibody [DT101 + BC199] - ab731 from Abcam

Prices $391.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone DT101 + BC199
Immunohistochemistry analysis of Melanoma tissue labeling MelanA with ab731.

Anti-MelanA antibody [EP1422Y] - ab51061 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1422Y
Overlay histogram showing Malme-3M cells stained with ab51061 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51061, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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