Anti-MelanA antibody [EP1422Y]

• Supplier: Abcam
• Catalog: ab51061
• Prices: $390.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1422Y
• Applications: WB IHC-P IHC-F FC
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues on the N-terminus of human MelanA
• Description: Rabbit Monoclonal
• Gene: MLANA
• Conjugate: Unconjugated

Product images

Overlay histogram showing Malme-3M cells stained with ab51061 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51061, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-MelanA antibody [EP1422Y] (ab51061) at 1/1000 dilution + Melanoma lysate at 10 µgSecondaryGoat anti-Rabbit HRP labeled at 1/2000 dilution

Ab51061 (1/100) staining human MelanA in melanoma tissue by immunohistochemistry using paraffin embedded tissue.Ab51061 (1/100) staining human MelanA in melanoma tissue by immunohistochemistry using paraffin embedded tissue.

Immunohistochemical analysis of Human malignant melanoma, staining MelanA with ab51061 at 1/20000 dilution. A Cy3-conjugated donkey anti-rabbit IgG (1/500) was used as the secondary antibody.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

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