Anti-Melanoma gp100 antibody [EP4863(2)]

• Supplier: Abcam
• Catalog: ab137078
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP4863(2)
• Applications: WB IHC-P ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide corresponding to residues in Human Melanoma gp100
• Description: Rabbit Monoclonal
• Gene: PMEL
• Conjugate: Unconjugated

Product images

Overlay histogram showing MALME 3M cells stained with ab137078 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137078, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Overlay histogram showing B16-F0 (Mouse skin melanoma cell) cells stained with ab137078 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab137078 at 1/450 dilution. The secondary antibody used was Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was Rabbit monoclonal IgG (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Melanoma gp100 antibody [EP4863(2)] (ab137078) at 1/1000 dilutionLane 1 : B16-F0 lysateLane 2 : Human melanoma lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labelling Melanoma gp100 with ab137078 at 1/100 dilution.

Product References

Critical residues in the PMEL/Pmel17 N-terminus direct the hierarchical assembly - Critical residues in the PMEL/Pmel17 N-terminus direct the hierarchical assembly

Leonhardt RM, Vigneron N, Hee JS, Graham M, Cresswell P. Mol Biol Cell. 2013 Apr;24(7):964-81.