PMEL

Gene Symbol PMEL
Entrez Gene 6490
Alt Symbol D12S53E, ME20, ME20-M, ME20M, P1, P100, PMEL17, SI, SIL, SILV, gp100
Species Human
Gene Type protein-coding
Description premelanosome protein
Other Description melanocyte protein PMEL|melanocyte protein Pmel 17|melanocyte protein mel 17|melanocytes lineage-specific antigen GP100|melanoma-associated ME20 antigen|melanosomal matrix protein17|silver locus protein homolog|silver, mouse, homolog of
Swissprots P40967 Q12763 B3KS57 Q16565 Q14448 B7Z6D7 Q14817
Accessions AAB00386 AAB19181 CAD10318 CCQ43382 EAW96852 EAW96853 EAW96854 EAW96855 P40967 AK092881 BAG52619 AK130180 AK300150 BAH13223 BC001414 AAH01414 BT007202 AAP35866 CA432578 DA035117 DA633173 EU176186 ABW03637 M32295 AAA35930 M77348 AAA60121 S73003 AAC60634 U01874 AAA18479 XM_006719569 XP_006719632 XM_011538685 XP_011536987 XM_011538686 XP_011536988 XM_011538687 XP_011536989 NM_001200053 NP_001186982 NM_001200054 NP_001186983 NM_006928 NP_008859
Function Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. {ECO:0000269|PubMed:11694580, ECO:0000269|PubMed:21962903}.
Subcellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus. Melanosome. Endosome, multivesicular body. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.
Tissue Specificity Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.

Pmel17 (E-7) - sc-377325 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pmel17 (E-7): sc-377325. Western blot analysis of Pmel17 expression in SK-MEL-28 whole cell lysate.

Pmel17 (C-2) - sc-393094 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pmel17 (C-2): sc-393094. Western blot analysis of Pmel17 expression in SK-MEL-28 whole cell lysate.

Pmel17 (H-300) - sc-33590 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Pmel17 (H-300): sc-33590. Western blot analysis of Pmel17 expression in CCRF-CEM (A) and Jurkat (B) whole cell lysates.

Pmel17 (K-18) - sc-15010 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pmel17 (K-18): sc-15010. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of germinal and non-germinal center cells.

Anti-Melanoma gp100 antibody - ab27435 from Abcam

Prices $378.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Paraffin embedded formalin fixed melanoma stained with anti melanoma gp100 (ab27435).

Anti-Melanoma gp100 antibody - ab116582 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Melanoma gp100 antibody (ab116582) at 1/100 dilution + CEM cell line lysate at 35 µg

Anti-Melanoma gp100 antibody - ab56095 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Melanoma gp100 antibody [3F272] - ab63297 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F272

Anti-Melanoma gp100 antibody [7E3] - ab117853 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7E3
Flow cytometry image using ab117853 at a 1/100 dilution, staining HEK293T cells transfected with Melanoma gp100 (Red), or an empty vector control plasmid (Blue).

Anti-Melanoma gp100 antibody [EP4863(2)] - ab137078 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EP4863(2)
Overlay histogram showing MALME 3M cells stained with ab137078 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137078, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Melanoma gp100 antibody [EPR4864] - ab137062 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4864
Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/10000 dilution (purified) + Human melanoma tissue lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Melanoma gp100 antibody [HMB45] - ab190713 from Abcam

Prices $379.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone HMB45
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling Melanoma gp100 at 1 µg/ml.

Anti-Melanoma gp100 antibody, prediluted - ab27428 from Abcam

Prices $378.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Ab27428, prediluted, staining Melanoma gp100 in formalin fixed, paraffin embedded human Melanoma sections by Immunohistochemistry.

Anti-PMEL / SILV / gp100 Antibody (clone 10E5) - LS-C114527 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10E5
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-SILV mouse monoclonal antibody. (Dilution 1:50).

Anti-PMEL / SILV / gp100 Antibody (Azide-free) - LS-C25588 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-PMEL / SILV / gp100 Antibody - LS-C25586 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-PMEL / SILV / gp100 Antibody (C-Terminus) - LS-C47016 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PMEL / SILV / gp100 Antibody (aa412-440, AP) - LS-C269436 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PMEL / SILV / gp100 Antibody - LS-C122119 from LifeSpan Bioscience

Prices $815.00
Sizes 1 ml
Host Mouse
Clonality Monoclonal

Anti-PMEL / SILV / gp100 Antibody (aa412-440, APC) - LS-C269437 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PMEL / SILV / gp100 Antibody (aa412-440, Biotin) - LS-C269438 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PMEL / SILV / gp100 Antibody (aa412-440, Azide-free, HRP) - LS-C269439 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PMEL / SILV / gp100 Antibody (aa412-440, FITC) - LS-C269440 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PMEL / SILV / gp100 Antibody (aa412-440, PE) - LS-C269441 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PMEL / SILV / gp100 Antibody (aa647-660) - LS-C55467 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (1 ug/ml) staining of Human Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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