Anti-Melanoma gp100 antibody [EPR4864]

• Supplier: Abcam
• Catalog: ab137062
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4864
• Applications: IHC-F WB IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)
• Description: Rabbit Monoclonal
• Gene: PMEL
• Conjugate: Unconjugated

Product images

Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/10000 dilution (purified) + Human melanoma tissue lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human melanoma with purified ab137062 at a working dilution of 1 in 400. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/1000 dilution (unpurified) + Human melanoma tissue lysate at 10 µgSecondaryHRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Immunohistochemical staining of paraffin embedded human melanoma with unpurified ab137062 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Unpurified ab137062 staining melanoma gp100 in human melanoma tissue sections by immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized and blocked with 4% horse serum + 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Magnification: 20X. Counterstained with DAPI.See Abreview

Immunohistochemical analysis using unpurified ab137062 showing negative staining in placenta tissue.

Immunohistochemical analysis using unpurifed ab137062 showing negative staining in normal skin tissue.

Immunohistochemical analysis using unpurified ab137062 showing negative staining in normal kidney tissue.

Immunohistochemical analysis using unpurified ab137062 showing negative staining in normal stomach tissue.