Metabotropic Glutamate Receptor 1a staining in rat brain (singulate cortex) using ab51314 at 1/100 dilution. Metabotropic Glutamate Receptor 1a detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color).
Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314) at 1/1000 dilution + Human brain lysate at 20 µgSecondaryAP-conjugated secondary at 1/5000 dilution
All lanes : Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314)Lane 1 : Rat cerebellar membranesLane 2 : Rat cerebellar membranes + DTT