Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]

• Supplier: Abcam
• Catalog: ab76316
• Prices: $398.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR2425Y
• Applications: WB IHC-P ICC/IF FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Metabotropic Glutamate Receptor 5 aa 1150 to the C-terminus (C terminal)
• Blocking Peptide: Metabotropic Glutamate Receptor 5 peptide
• Description: Rabbit Monoclonal
• Gene: GRM5
• Conjugate: Unconjugated

Product images

ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde and samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/2000) for 16 hours at 21°C. An alexa fluor® 594 conjugated goat anti-rabbit IgG secondary was used at 1/500.See Abreview

See Abreview

ab76316 staining Metabotropic Glutmate Receptor 5 in rat hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 24 hours at 4°C. An Alexa Fluor® 594-conjugated chicken anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview

Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1/10000 dilution + mouse brain lysate at 10 µgSecondaryGoat anti-rabbit-HRP at 1000 developed using the ECL technique

ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry. Tissue was fixed with formaldehyde and citrate-mediated antigen retrieval was performed. Samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/1000) for 2 hours at 21°C. A biotin conjugated goatanti-rabbit IgG secondary was used at 1/250.See Abreview

Human brain tissue with ab76316 at 1/250 dilution.

Product References

Neuregulin 1 signalling modulates mGluR1 function in mesencephalic dopaminergic - Neuregulin 1 signalling modulates mGluR1 function in mesencephalic dopaminergic

Ledonne A, Nobili A, Latagliata EC, Cavallucci V, Guatteo E, Puglisi-Allegra S, D'Amelio M, Mercuri NB. Mol Psychiatry. 2014 Sep 30.

Compensatory molecular and functional mechanisms in nervous system of the - Compensatory molecular and functional mechanisms in nervous system of the

Rossi PI, Musante I, Summa M, Pittaluga A, Emionite L, Ikehata M, Rastaldi MP, Ravazzolo R, Puliti A. Cereb Cortex. 2013 Sep;23(9):2179-89.

PKC phosphorylation regulates mGluR5 trafficking by enhancing binding of Siah-1A. - PKC phosphorylation regulates mGluR5 trafficking by enhancing binding of Siah-1A.

Ko SJ, Isozaki K, Kim I, Lee JH, Cho HJ, Sohn SY, Oh SR, Park S, Kim DG, Kim CH, Roche KW. J Neurosci. 2012 Nov 14;32(46):16391-401.