![All lanes : Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953) at 1/1000 dilutionLane 1 : HepG2 cell lysatesLane 2 : 293T cell lysatesLane 3 : HeLa cell lysatesLane 4 : C6 cell lysatesLane 5 : RAW 264.7 cell lysatesLane 6 : PC12 cell lysatesLane 7 : NIH 3T3 cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/22044_Methionine-Aminopeptidase-2-Primary-antibodies-ab124953-1.jpg)
All lanes : Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953) at 1/1000 dilutionLane 1 : HepG2 cell lysatesLane 2 : 293T cell lysatesLane 3 : HeLa cell lysatesLane 4 : C6 cell lysatesLane 5 : RAW 264.7 cell lysatesLane 6 : PC12 cell lysatesLane 7 : NIH 3T3 cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution
ab124953 at 1/10 dilution staining Methionine Aminopeptidase 2 in permeabilized 293T cells by Flow cytometry (red) or a Rabbit IgG (negative control) (green).
ab124953 staining Methionine Aminopeptidase 2 in Mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with Acetone and blocked with 5% Serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/25 in PBS + 5% goat serum) for 1 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.