Flow cytometry analysis of 2% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1 with ab185540 at 1/180 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
Immunofluorescent analysis of 4% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Counterstained with Dapi blue.
Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling Methionyl Aminopeptidase 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Methionyl Aminopeptidase 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Methionyl Aminopeptidase 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.
All lanes : Anti-Methionyl Aminopeptidase 1 antibody [EPR15452(B)] (ab185540) at 1/1000 dilutionLane 1 : A375 cell lysateLane 2 : HUVEC cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution