Anti-MGEA5 antibody
| Name | Anti-MGEA5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab105217 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rabbit, Horse, Bovine, Dog, Pig, Monkey, Ape |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human MGEA5 |
| Description | Rabbit Polyclonal |
| Gene | MGEA5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MGEA5 antibody (ab105217) at 1 µg/mlLane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell LysateLane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell LysateLane 3 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 5 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 6 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate with Immunising peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab105217 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab105217 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.