MGEA5

Gene Symbol MGEA5
Entrez Gene 10724
Alt Symbol MEA5, NCOAT, OGA
Species Human
Gene Type protein-coding
Description meningioma expressed antigen 5 (hyaluronidase)
Other Description N-acetyl-beta-D-glucosaminidase|N-acetyl-beta-glucosaminidase|O-GlcNAcase|beta-N-acetylglucosaminidase|beta-N-acetylhexosaminidase|beta-hexosaminidase|bifunctional protein NCOAT|hyaluronidase in meningioma|meningioma-expressed antigen 5|nuclear cytoplasmic O-GlcNAcase and acetyltransferase|protein O-GlcNAcase
Swissprots Q9HAR0 O60502 Q86WV0 Q9BVA5 E9PGF9 O75166 B7WPB9 D3DR79 Q8IV98
Accessions EAW49741 EAW49742 EAW49743 EAW49744 O60502 AB014579 BAA31654 AF036144 AAD05385 AF307332 AAG21428 AK000988 AK002091 BAG51014 AK027008 AK302626 BAG63869 BC001343 AAH01343 BC014201 BC032591 BC039583 AAH39583 BC047877 AAH47877 BQ016159 DC353958 XM_011539180 XP_011537482 XM_011539181 XP_011537483 XM_011539182 XP_011537484 XR_246060 XR_945587 XR_945588 NM_001142434 NP_001135906 NM_012215 NP_036347
Function Isoform 1: Cleaves GlcNAc but not GalNAc from O- glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4- methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714). {ECO:0000269|PubMed:11148210, ECO:0000269|PubMed:11788610, ECO:0000269|PubMed:20673219, ECO:0000269|PubMed:22365600, ECO:0000269|PubMed:24088714}.
Subcellular Location Isoform 3: Nucleus {ECO:0000269|PubMed:11341771}.
Tissue Specificity Ubiquitous. Shows highest expression in the brain, placenta and pancreas. {ECO:0000269|PubMed:11148210, ECO:0000269|PubMed:11341771}.

NCOAT (G-12) - sc-376429 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCOAT (G-12): sc-376429. Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of squamous epithelial cells.

NCOAT (H-300) - sc-135093 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NCOAT (H-300): sc-135093. Western blot analysis of NCOAT expression in SHP-77 whole cell lysate.

NCOAT (L-14) - sc-66612 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-MGEA5 antibody - ab105217 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MGEA5 antibody (ab105217) at 1 µg/mlLane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell LysateLane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell LysateLane 3 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 5 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 6 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate with Immunising peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MGEA5 antibody - ab197389 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MGEA5 antibody (ab197389) at 1/300 dilution + human fetal liver tissue lysate at 40 µg

Anti-MGEA5 antibody - ab187701 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of MGEA5 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab187701 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-MGEA5 antibody - ab68522 from Abcam

Prices $387.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-MGEA5 antibody (ab68522) at 1/2000 dilution + 293T Cell Lysate at 10 µg with 3% MilkSecondaryHRP conjugated Rat anti-mouse polyclonal at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MGEA5 antibody [EPR7154(B)] - ab124807 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7154(B)
All lanes : Anti-MGEA5 antibody [EPR7154(B)] (ab124807) at 1/10000 dilutionLane 1 : HeLa lysateLane 2 : BxPC-3 lysateLane 3 : JAR lysateLane 4 : Fetal brain lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Beta-N-Acetylglucosaminidase Antibody (aa6-240, Biotin) - LS-C299433 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MGEA5 Antibody (aa500-550) - LS-C290982 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human MGEA5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MGEA5 antibody used for IP at 6 ug per reaction. MGEA5 was also immunoprecipitated by rabbit anti-MGEA5 antibody BL15772. For blotting immunoprecipitated MGEA5, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-Beta-N-Acetylglucosaminidase Antibody (aa319-450, Biotin) - LS-C299935 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Beta-N-Acetylglucosaminidase Antibody (aa6-240) - LS-C295533 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant O-GlcNAcase / MGEA5.

Anti-Beta-N-Acetylglucosaminidase Antibody (aa6-240, FITC) - LS-C305062 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Beta-N-Acetylglucosaminidase Antibody (aa319-450) - LS-C295538 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant O-GlcNAcase / MGEA5.

Anti-Beta-N-Acetylglucosaminidase Antibody (aa319-450, FITC) - LS-C304100 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MGEA5 Antibody - LS-C308997 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-MGEA5 Antibody (Internal, clone EPR7154(B)) - LS-C138942 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7154(B)
IHC of paraffin-embedded human kidney tissue using anti-MGEA5 antibody.

Anti-MGEA5 Antibody - LS-C311392 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MGEA5 Antibody (aa823-916) - LS-C308999 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

O-GlcNAcase/OGA/MGEA5 Antibody (EPR7154(B)) - NBP1-96679 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7154(B)
Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody (EPR7154(B)) [NBP1-96679] - (A) HeLa, (B) BxPC-3, © JAR, and (D) fetal brain lysates.

O-GlcNAcase/OGA/MGEA5 Antibody - NBP1-81244 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

O-GlcNAcase/OGA/MGEA5 Antibody - H00010724-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody [H00010724-B01P] - Analysis of MGEA5 expression in transfected 293T cell line by MGEA5 polyclonal antibody. Lane1:MGEA5 transfected lysate(100.76 KDa). Lane2:Non-transfected lysate.

O-GlcNAcase/OGA/MGEA5 Antibody - NBP2-32233 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- MGEA5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

O-GlcNAcase/OGA/MGEA5 Antibody - H00010724-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

O-GlcNAcase/OGA/MGEA5 Antibody (1C7) - H00010724-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C7
Immunocytochemistry/Immunofluorescence: O-GlcNAcase/OGA/MGEA5 Antibody (1C7) [H00010724-M02] - Analysis of monoclonal antibody to MGEA5 on HeLa cell. Antibody concentration 10 ug/ml.
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