Anti-MGMT antibody
| Name | Anti-MGMT antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab69629 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human, Rat |
| Antigen | Synthetic peptide derived from the internal region of human MGMT |
| Description | Rabbit Polyclonal |
| Gene | MGMT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MGMT antibody (ab69629) at 1/500 dilutionLane 1 : Extracts from Jurkat cells.Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
ab69629 staining MGMT in T98G human glioma cell line by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 3% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 20°C. The secondary antibody was Alexa Fluor® 555-conjugated goat anti-rabbit polyclonal, diluted 1/300. MGMT staining is shown in red (AF 555) and stains nuclei and to a small extent, cytoplasm. Nuclei were stained with DAPI (blue), while microtubules were stained using an anti-tubulin FITC conjugate (green).See Abreview