MGMT

Gene Symbol MGMT
Entrez Gene 4255
Alt Symbol -
Species Human
Gene Type protein-coding
Description O-6-methylguanine-DNA methyltransferase
Other Description 6-O-methylguanine-DNA methyltransferase|O-6-methylguanine-DNA-alkyltransferase|O6-methylguanine-DNA methyltransferase|methylated-DNA--protein-cysteine methyltransferase|methylguanine-DNA methyltransferase
Swissprots Q5VY78 P16455
Accessions AAD13867 AGQ03778 EAW49166 EAW49167 P16455 AK293593 BAG57059 BC000824 AAH00824 BI520029 BT006714 AAP35360 DC387686 DQ890723 ABM81649 DQ893901 ABM84827 KC249995 AGQ03777 M29971 AAA59596 M31767 AAA52317 M60761 AAA59594 X54228 CAA38137 XM_005252682 XP_005252739 XM_006717863 XP_006717926 XM_011539817 XP_011538119 NM_002412 NP_002403
Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Subcellular Location Nucleus.

MGMT Antibody - 2739 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat and MOLT4 cells using MGMT Antibody.

MGMT (C-5) - sc-271154 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MGMT (C-5): sc-271154. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in glomeruli and tubules.

MGMT (G-10) - sc-514277 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MGMT (G-10): sc-514277. Western blot analysis of MGMT expression in non-transfected 293T: sc-117752 (A), human MGMT transfected 293T: sc-159668 (B), MCF7 (C), Hep G2 (D) and Caco-2 (E) whole cell lysates.

MGMT (C-20) - sc-8825 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MGMT (C-20): sc-8825. Western blot analysis of MGMT expression in MCF7 nuclear extract.

MGMT (D-4) - sc-166527 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MGMT (D-4): sc-166527. Western blot analysis of MGMT expression in non-transfected: sc-110760 (A) and human MGMT transfected: sc-110499 (B) 293 whole cell lysates and MCF7 nuclear extract (C).

MGMT (E-1) - sc-166528 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MGMT (E-1): sc-166528. Western blot analysis of MGMT expression in non-transfected: sc-110760 (A) and human MGMT transfected: sc-110499 (B) 293 whole cell lysates and MCF7 nuclear extract (C).

MGMT (F-5) - sc-271155 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MGMT (F-5): sc-271155. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear staining of glomerular cells.

MGMT (E-6) - sc-166491 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MGMT (E-6): sc-166491. Western blot analysis of MGMT expression in MCF7 whole cell lysate.

MGMT (FL-207) - sc-28241 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MGMT (FL-207): sc-28241. Western blot analysis of MGMT expression in non-transfected: sc-110760 (A) and human MGMT transfected: sc-110499 (B) 293 whole cell lysates.

MGMT (MT 3.1) - sc-56157 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MGMT (MT 3.1): sc-56157. Western blot analysis of MGMT expression in non-transfected: sc-110760 (A) and human MGMT transfected: sc-110499 (B) 293 whole cell lysates.

MGMT (N-16) - sc-8824 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MGMT (N-16): sc-8824. Western blot analysis of MGMT expression in non-transfected: sc-110760 (A) and human MGMT transfected: sc-110499 (B) 293 whole cell lysates.

MGMT (MT5.1) - sc-33674 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MGMT (MT5.1): sc-33674. Western blot analysis of MGMT expression in non-transfected 293: sc-110760 (A) and human MGMT transfected 293: sc-110499 (B) whole cell lysates and MCF7 nuclear extract (C).

MGMT (Y-16) - sc-8826 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MGMT (Y-16): sc-8826. Western blot analysis of MGMT expression in non-transfected: sc-110760 (A) and human MGMT transfected: sc-110499 (B) 293 whole cell lysates.

MGMT (SPM287) - sc-56432 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MGMT (SPM287): sc-56432. Western blot analysis of MGMT expression in Jurkat (A) and MCF7 (B) whole cell lysates.

Anti-MGMT antibody - ab69629 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MGMT antibody (ab69629) at 1/500 dilutionLane 1 : Extracts from Jurkat cells.Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-MGMT antibody - ab112504 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MGMT antibody (ab112504)Lane 1 : Rat brain tissue lysateLane 2 : Rat liver tissue lysateLane 3 : Rat spleen tissue lysateLane 4 : Rat thymus tissue lysateLane 5 : MCF-7 whole cell lysateLane 6 : HeLa whole cell lysateLane 7 : Jurkat whole cell lysateLane 8 : Raji whole cell lysate

Anti-MGMT antibody - ab80513 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of MGMT staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80513, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MGMT antibody - ab55442 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
MGMT antibody (ab55442) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.

Anti-MGMT antibody [EPR4397] - ab108630 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4397
All lanes : Anti-MGMT antibody [EPR4397] (ab108630) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : Jurkat ell lysateLane 3 : HeLa cell lysateLane 4 : HT29 cell lysateLysates/proteins at 10 µg per lane.

Anti-MGMT antibody [EPR4398] - ab108989 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4398
All lanes : Anti-MGMT antibody [EPR4398] (ab108989) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-MGMT antibody [MT 3.1] - ab81705 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone MT 3.1
Formalin fixed, paraffin embedded human colon carcinoma stained with anti MGMT (form of ab81705 containing BSA and azide). Peroxidase conjugate and AEC chromogen were used. Note nuclear staining of tumor cells.

Anti-MGMT antibody [MT3.1] - ab39253 from Abcam

Prices $398.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone MT3.1
ab39253 staining MGMT in Human stomach tissue sections by IHC-Fr (frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.See Abreview

Anti-MGMT antibody [SPM287], prediluted - ab54306 from Abcam

Prices $376.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone SPM287
Overlay histogram showing Jurkat cells stained with ab54306 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54306, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-MGMT antibody - C-terminal - ab85238 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-MGMT antibody - C-terminal (ab85238) at 1/50 dilution + CEM cell line lysate at 35 µg

Anti-MGMT antibody - C-terminal - ab200147 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-MGMT antibody - C-terminal (ab200147) at 1/500 dilutionLane 1 : Mouse thymus total cell extractLane 2 : HeLa total cell extractLane 3 : Jurkat total cell extract
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