All lanes : Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/1000 dilutionLane 1 : K562 cell lysatesLane 2 : HeLa cell lysatesLane 3 : Caco2 cell lysatesLane 4 : NCCIT cell lysatesLane 5 : Human brain lysatesLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit at 1/2000 dilution
ab124929, at 1/250 dilution, staining Mib1/Mindbomb in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.
Immunohistochemical analysis of Human breast cancer tissue, staining Mib1/Mindbomb with ab124929.Tissue was fixed with paraformaldehyde and permeabilized with Tween buffer for 30 minutes at 20°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 9). Samples were incubated with primary antibody (1/5000 in diluent) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody. See Abreview
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD