Anti-MICA antibody (ab93170) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg
ab93170, at a 1/10 dilution, staining MICA in SK-Br-3 cells by flow cytometric analysis (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibody used for the analysis.
ab93170, at a 1/50 dilution, staining MICA in formalin-fixed and paraffin-embedded Human hepatocarcinoma peroxidase-conjugated to FITC-conjugated goat-anti-rabbit secondary antibody, followed by DAB staining.
ICC/IF image of ab93170 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93170, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-MICA antibody (ab93170) at 1/500 dilution + Human MICA full length protein (ab73819) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute