All lanes : Anti-MICAL1 antibody [EPR11486] (ab181145) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : Human fetal lung lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-MICAL1 antibody [EPR11486] (ab181145) at 1/10000 dilutionLane 1 : A549 cell lysateLane 2 : Human fetal kidney lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181145 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.