Anti-MIF antibody [EPR12463]

• Supplier: Abcam
• Catalog: ab175189
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR12463
• Applications: WB IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MIF aa 50 to the C-terminus (Cysteine residue)
• Description: Rabbit Monoclonal
• Gene: MIF
• Conjugate: Unconjugated

Product images

All lanes : Anti-MIF antibody [EPR12463] (ab175189) at 1/1000 dilution (unpurified)Lane 1 : Y79 cell lysateLane 2 : Mouse brain tissue lysateLane 3 : Rat brain tissue lysateLysates/proteins at 20 µg/ml per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-MIF antibody [EPR12463] (ab175189) at 1/2000 dilution (purified)Lane 1 : Y79 cell lysateLane 2 : Mouse brain tissue lysateLane 3 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-MIF antibody [EPR12463] (ab175189) at 1/1000 dilution (unpurified)Lane 1 : Y79 lysatesLane 2 : THP-1 lysatesLane 3 : Molt-4 lysatesLane 4 : Human fetal brain lysatesLysates/proteins at 10 µg per lane.

Flow cytometry analysis of THP-1 cells labelling MIF with unpurified ab175189 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary anitbody. A rabbit monoclonal IgG was used as the isotype control (green).

Flow cytometry analysis of THP-1 cells labelling MIF with purified ab175189 at 1/140 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary anitbody. A rabbit monoclonal IgG was used as the isotype control (green).

Flow cytometric analysis of permeabilized Molt-4 cells using unpurified ab175189 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

ab175189 (unpurified) at 1/30 immunoprecipitating MIF in human fetal brain. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

ab175189 (purified) at 1/50 immunoprecipitating MIF in human fetal brain. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.