MIF

Gene Symbol MIF
Entrez Gene 4282
Alt Symbol GIF, GLIF, MMIF
Species Human
Gene Type protein-coding
Description macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Other Description L-dopachrome isomerase|L-dopachrome tautomerase|macrophage migration inhibitory factor|phenylpyruvate tautomerase
Swissprots Q6FHV0 B2R4S3 A5Z1R8 P14174 Q2V4Y5
Accessions AAA21814 ABB96245 EAW59620 P14174 AF469046 AAL78635 AK311929 BAG34870 BC000447 AAH00447 BC007376 BC007676 AAH07676 BC008914 AAH08914 BC013976 AAH13976 BC022414 AAH22414 BC051440 BC053376 AAH53376 BT007148 AAP35812 CR407644 CAG28572 CR456520 CAG30406 CR541651 CAG46452 CU013120 CAK54551 CU013408 CAK54850 DQ892195 ABM83121 DQ895390 ABM86316 JQ846015 AFM09726 L10612 AAA35892 M25639 AAA36315 M95775 AAA36179 Z23063 CAA80598 NM_002415 NP_002406
Function Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. {ECO:0000269|PubMed:15908412, ECO:0000269|PubMed:17443469, ECO:0000269|PubMed:23776208}.
Subcellular Location Secreted. Cytoplasm. Note=Does not have a cleavable signal sequence and is secreted via a specialized, non- classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
Top Pathways Phenylalanine metabolism, Tyrosine metabolism

MIF (11) - sc-130329 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MIF (11): sc-130329. Western blot analysis of MIF expression in non-transfected: sc-117752 (A) and human MIF transfected: sc-129140 (B) 293T whole cell lysates.

MIF (D-2) - sc-271631 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MIF (D-2): sc-271631. Western blot analysis of MIF expression in Jurkat whole cell lysate.

MIF (FL-115) - sc-20121 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MIF (FL-115): sc-20121. Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic and membrane staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

MIF (N-18) - sc-48241 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MIF (N-18): sc-48241 Western blot analysis of MIF expression in Jurkat (A), AML-193 (B), THP-1 (C) and RAW 264.7 (D) whole cell lysates.

MIF (C-13) - sc-47159 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MIF (N-20) - sc-16965 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MIF (N-20): sc-16965. Western blot analysis of MIF expression in U-698 whole cell lysate.

MIF (S-18) - sc-16966 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MIF (4E4) - sc-53915 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
MIF (4E4): sc-53915. Western blot analysis of MIF expression in HL-60 (A), Jurkat (B), AML-193 (C) and THP-1 (D) whole cell lysates.

Anti-MIF antibody - ab55445 from Abcam

Prices $400.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
MIF antibody (ab55445) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lung, adenosqumous cell carcinoma tissue.

Anti-MIF antibody - ab65869 from Abcam

Prices $387.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This image shows rat kidney tissue stained with ab65869 at 2µg/ml. Cytoplasmic staining of MIF, using DAB.

Anti-MIF antibody - ab14574 from Abcam

Prices $370.00
Sizes 25 µg
Host Goat
Clonality Polyclonal

Anti-MIF antibody - ab131093 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MIF antibody [2Ar3] - ab14575 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2Ar3

Anti-MIF antibody [4E4] - ab118852 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4E4
Anti-MIF antibody [4E4] (ab118852) at 1/1000 dilution + HL60 cell extractSecondarygoat anti-mouse conjugated to HRPdeveloped using the ECL technique

Anti-MIF antibody [EPR12463] - ab175189 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12463
All lanes : Anti-MIF antibody [EPR12463] (ab175189) at 1/1000 dilution (unpurified)Lane 1 : Y79 cell lysateLane 2 : Mouse brain tissue lysateLane 3 : Rat brain tissue lysateLysates/proteins at 20 µg/ml per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-MIF antibody [EPR12463] (Alexa Fluor® 488) - ab197509 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12463
ab197509 staining MIF in Jurkat cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab197509 at 1/50 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-MIF antibody [EPR12463] (Alexa Fluor® 647) - ab196933 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12463
Overlay histogram showing Jurkat cells stained with ab196933 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196933, 1/500 dilution) for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.This antibody gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MIF antibody [EPR12463] (HRP) - ab196645 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12463
All lanes : Anti-MIF antibody [EPR12463] (HRP) (ab196645) at 1/2000 dilutionLane 1 : Y79 (Human retinoblastoma cell line) Whole Cell LysateLane 2 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-MIF Antibody (aa2-32) - LS-C59678 from LifeSpan Bioscience

Prices $395.00
Sizes 500 µg
Host Chicken
Clonality Polyclonal
Anti-Human MIF Polyclonal Antibody - Western Blot. Western blot of IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 ng of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kD. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Chicken Rabbit IgG [H&L] (code for 1 h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Anti-MIF Antibody (aa67) - LS-C190226 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MIF Antibody (HRP) - LS-C211834 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MIF Antibody - LS-C211836 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MIF Antibody (FITC) - LS-C211837 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MIF Antibody (aa35-84) - LS-C117269 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MIF antibody LS-C117269 Western blot of Fetal Heart lysate.

Anti-MIF Antibody (Biotin) - LS-C211838 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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