Anti-MIF antibody [EPR12463] (HRP)

• Supplier: Abcam
• Catalog: ab196645
• Prices: $425.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR12463
• Applications: IHC-P WB
• Species Reactivities: Human, Mouse, Rat
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MIF aa 50 to the C-terminus (Cysteine residue)
• Description: Rabbit Monoclonal
• Gene: MIF
• Conjugate: HRP

Product images

All lanes : Anti-MIF antibody [EPR12463] (HRP) (ab196645) at 1/2000 dilutionLane 1 : Y79 (Human retinoblastoma cell line) Whole Cell LysateLane 2 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

IHC image of MIF staining in a section of formalin-fixed paraffin-embedded normal human lung, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196645 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.