Anti-Mineralocorticoid Receptor antibody

• Supplier: Abcam
• Catalog: ab64457
• Prices: $385.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Rat, Xenopus, Primate
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human Mineralocorticoid Receptor
• Description: Rabbit Polyclonal
• Gene: NR3C2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Mineralocorticoid Receptor antibody (ab64457) at 1 µg/mlLane 1 : Kidney (Mouse) Tissue Lysate Lane 2 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lane 3 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

ICC/IF image of ab64457 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.

IHC image of Mineralocorticoid Receptor staining in Human Tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64457, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Product References

Selective ablation of glucocorticoid receptor in mouse keratinocytes increases - Selective ablation of glucocorticoid receptor in mouse keratinocytes increases

Latorre V, Sevilla LM, Sanchis A, Perez P. J Invest Dermatol. 2013 Dec;133(12):2771-9.

Reverse effect of mammalian hypocalcemic cortisol in fish: cortisol stimulates - Reverse effect of mammalian hypocalcemic cortisol in fish: cortisol stimulates

Lin CH, Tsai IL, Su CH, Tseng DY, Hwang PP. PLoS One. 2011;6(8):e23689.