Anti-Mineralocorticoid Receptor antibody [H3122]
| Name | Anti-Mineralocorticoid Receptor antibody [H3122] |
|---|---|
| Supplier | Abcam |
| Catalog | ab41912 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | H3122 |
| Applications | ELISA IP IHC-P IHC-F WB FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Bird, Monkey, Marmoset |
| Antigen | Baculovirus-expressed recombinant fragment: ETKGYHSLPE GLDMERRWGQ VSQAVERSSL GPTERTDENN YMEIVNVSCV SGAIPNNSTQ GSSKEKQELL PCLQQDNNRP GILTSDIKTE LESKELSA, corresponding to amino acids 2-99 of Human Mineralocorticoid Receptor |
| Description | Mouse Monoclonal |
| Gene | NR3C2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab41912 staining Mineralocorticoid in male European Starling brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were transcardially perfused with paraformaldehyde, and immersed in paraformaldyhyde for 24 hours and then 30% sucrose for 24 hours. Slices were cut at a 40 micron thickness. Tissue samples were permeablized with Triton and blocked with 10% serum for 1 hour at 21°C. The sample was incubated with primary antibody (1/500) for 24 hours. A biotin-conjugated Goat anti-mouse IgG monoclonal (1/400) was used as the secondary antibody.See Abreview
ab41912 staining Mineralocorticoid Receptor in human large intestine paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
ICC/IF image of ab41912 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41912, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab41912 used in Flow Cytometry.Human peripheral blood leukocyte cells were fixed with paraformaldehyde, permeabilized with saponin and then stained with ab41912 (which had been directly conjugated to Alexa Fluor 647), used at a 1/50 dilution. Gated on CD3+ lymphocytes (T cells).See Abreview
Product References
Spironolactone attenuates bleomycin-induced pulmonary injury partially via - Spironolactone attenuates bleomycin-induced pulmonary injury partially via
Ji WJ, Ma YQ, Zhou X, Zhang YD, Lu RY, Guo ZZ, Sun HY, Hu DC, Yang GH, Li YM, Wei LQ. PLoS One. 2013 Nov 19;8(11):e81090.
Deletion of cardiomyocyte mineralocorticoid receptor ameliorates adverse - Deletion of cardiomyocyte mineralocorticoid receptor ameliorates adverse
Fraccarollo D, Berger S, Galuppo P, Kneitz S, Hein L, Schutz G, Frantz S, Ertl G, Bauersachs J. Circulation. 2011 Feb 1;123(4):400-8.
Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes. - Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes.
Lopez-Andres N, Inigo C, Gallego I, Diez J, Fortuno MA. Endocrinology. 2008 Oct;149(10):4970-8.