Anti-MiTF antibody [C5]
| Name | Anti-MiTF antibody [C5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab80651 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | C5 |
| Applications | ICC/IF ICC/IF IHC-P FC WB EMSA |
| Species Reactivities | Mouse, Rat, Horse, Dog, Human |
| Antigen | N-terminal fragment of human MiTF protein |
| Description | Mouse Monoclonal |
| Gene | MITF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab80651 staining MiTF in human melanoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and and DAB chromogen. Note nuclear staining of tumor cells. Staining was performed using antibody containing BSA and azide.
![Overlay histogram showing Malme-3M cells stained with ab80651 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80651, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3M cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/22802_MiTF-Primary-antibodies-ab80651-2.jpg)
Overlay histogram showing Malme-3M cells stained with ab80651 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80651, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3M cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Senescent cells develop a PARP-1 and nuclear factor-{kappa}B-associated secretome - Senescent cells develop a PARP-1 and nuclear factor-{kappa}B-associated secretome
Ohanna M, Giuliano S, Bonet C, Imbert V, Hofman V, Zangari J, Bille K, Robert C, Bressac-de Paillerets B, Hofman P, Rocchi S, Peyron JF, Lacour JP, Ballotti R, Bertolotto C. Genes Dev. 2011 Jun 15;25(12):1245-61.
Age-resolving osteopetrosis: a rat model implicating microphthalmia and the - Age-resolving osteopetrosis: a rat model implicating microphthalmia and the
Weilbaecher KN, Hershey CL, Takemoto CM, Horstmann MA, Hemesath TJ, Tashjian AH, Fisher DE. J Exp Med. 1998 Mar 2;187(5):775-85.
MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in - MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in
Hemesath TJ, Price ER, Takemoto C, Badalian T, Fisher DE. Nature. 1998 Jan 15;391(6664):298-301.