MITF

Gene Symbol MITF
Entrez Gene 4286
Alt Symbol CMM8, MI, WS2, WS2A, bHLHe32
Species Human
Gene Type protein-coding
Description microphthalmia-associated transcription factor
Other Description class E basic helix-loop-helix protein 32
Swissprots Q9P2Y8 B4DJL2 Q14841 E9PFN0 O75030 Q9P2V1 D3K197 Q9P2V0 Q9P2V2
Accessions AAC39639 AIZ97493 BAA95206 BAA95207 BAA95208 BAA95209 EAW65479 EAW65480 EAW65481 EAW65482 EAW65483 EAW65484 O75030 AB006909 BAA32288 AB006988 BAB83926 AB006989 BAC16749 AB061771 BAB85121 AK291318 BAF84007 AK296129 BAG58874 AK297858 BAG60189 AL110195 CAB53672 AL117653 AW242257 AY632572 BC011461 BC012503 BC026961 AAH26961 BC065243 AAH65243 BM800230 BQ219650 BU167035 DA058963 DC388606 GU355676 ADB90411 Z29678 CAA82775 XM_005264754 XP_005264811 XM_005264755 XP_005264812 XM_006713164 XP_006713227 XM_011533722 XP_011532024 XM_011533723 XP_011532025 XM_011533724 XP_011532026 XM_011533725 XP_011532027 XM_011533726 XP_011532028 NM_000248 NP_000239 NM_001184967 NP_001171896 NM_001184968 NP_001171897 NM_006722 NP_006713 NM_198158 NP_937801 NM_198159 NP_937802 NM_198177 NP_937820 NM_198178 NP_937821
Function Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. {ECO:0000269|PubMed:10587587, ECO:0000269|PubMed:22647378}.
Subcellular Location Nucleus.
Tissue Specificity Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines. {ECO:0000269|PubMed:20163701}.
Top Pathways Melanoma, Melanogenesis, Osteoclast differentiation

MITF (D5G7V) Rabbit mAb - 12590 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5G7V
Western blot analysis of extracts from various cell lines using MITF (D5G7V) Rabbit mAb.

MITF (N-15) - sc-10999 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MITF (N-15): sc-10999. Western blot analysis of MITF expression in C32 (A) and NIH/3T3 (B) nuclear extracts.

MITF (H-50) - sc-25386 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MITF (H-50): sc-25386. Western blot analysis of MITF expression in non-transfected: sc-117752 (A) and human MITF transfected: sc-113752 (B) 293T whole cell lysates.

MITF (C-17) - sc-11002 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MITF (21D1418) - sc-52938 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
MITF (21D1418): sc-52938. Western blot analysis of MITF expression in Jurkat (A) and SK-MEL-28 (B) nuclear extracts.

MITF (SPM290) - sc-56433 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
MITF (SPM290): sc-56433. Western blot analysis of MITF expression in non-transfected: sc-117752 (A) and human MITF transfected: sc-114536 (B) 293T whole cell lysates.

MITF (D5) - sc-56726 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
MITF (D5): sc-56726. Western blot analysis of MITF expression in non-transfected: sc-117752 (A), human MITF transfected: sc-113569 (B) and human MITF transfected: sc-113752 (C) 293T whole cell lysates.

MITF (4H205) - sc-71587 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
MITF (4H205): sc-71587. Western blot analysis of MITF expression in Jurkat nuclear extract.

MITF (C5) - sc-56725 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
MITF (C5): sc-56725. Western blot analysis of MITF expression in non-transfected: sc-117752 (A) and human MITF transfected: sc-114536 (B) 293T whole cell lysates.

MITF (3F276) - sc-71588 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
MITF (3F276): sc-71588. Western blot analysis of MITF expression in non-transfected: sc-117752 (A) and human MITF transfected: sc-113569 (B) 293T whole cell lysates.

Anti-MiTF antibody - ab20663 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MiTF antibody (ab20663) at 1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : HeLa nuclear lysateLane 3 : A431 whole cell lysateLane 4 : HeLa whole cell lysate with Human MiTF peptide (ab21454) at 1 µg/mlLane 5 : HeLa nuclear lysate with Human MiTF peptide (ab21454) at 1 µg/mlLane 6 : A431 whole cell lysate with Human MiTF peptide (ab21454) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

Anti-MiTF antibody - ab59232 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab59232 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59232, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MiTF antibody [21D1418] - ab13703 from Abcam

Prices $382.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 21D1418

Anti-MiTF antibody [34CA5] - ab49387 from Abcam

Prices $378.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 34CA5
ab49387 staining MiTF (brown) in normal human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation in a buffer pH 9. Samples were incubated with primary antibody (1/25 in PBS + 3% BSA) for 1 hour. An undiluted biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody. Melanocytes stained violet.See Abreview

Anti-MiTF antibody [C5] - ab80651 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone C5
ab80651 staining MiTF in human melanoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and and DAB chromogen. Note nuclear staining of tumor cells. Staining was performed using antibody containing BSA and azide.

Anti-MiTF antibody [C5] (FITC) - ab150195 from Abcam

Prices $394.00
Sizes 25 µl
Host Mouse
Clonality Monoclonal
Clone C5

Anti-MiTF antibody [C5] - ChIP Grade - ab12039 from Abcam

Prices $403.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone C5
Anti-MiTF antibody [C5] - ChIP Grade (ab12039) at 1 µg/ml + Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MiTF antibody [D5] - ab3201 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone D5
ICC/IF image of ab3201 stained Malme-3M cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab3201 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-MiTF antibody [EPR9731] - ab140606 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9731
All lanes : Anti-MiTF antibody [EPR9731] (ab140606) at 1/1000 dilutionLane 1 : A375 cell lysateLane 2 : F0 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-MiTF antibody - ChIP Grade - ab122982 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab122982, staining MiTF in the RPE (Retinal Pigment Epithelium) in chicken embryo (stage 14) by Immunofluorescence.

Anti-MiTF antibody - N-terminal - ab170466 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-MiTF antibody - N-terminal (ab170466) + K562 cell lysate at 35 µg

Anti-MiTF antibody - N-terminal - ab199929 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-MiTF antibody - N-terminal (ab199929) at 1/1000 dilutionLane 1 : Mouse brain total cell extractLane 2 : Mouse thymus total cell extract

Anti-MITF Antibody (clone D5) - LS-C88472 from LifeSpan Bioscience

Prices $275.00, $520.00, $805.00
Sizes 20 µg, 500 µl, 200 µg
Host Mouse
Clonality Monoclonal
Clone D5
Formalin-fixed, paraffin-embedded human melanoma stained with peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Anti-MITF Antibody (aa170-279) - LS-C189101 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-MITF Antibody (clone C5/D5) - LS-C202882 from LifeSpan Bioscience

Prices $445.00
Sizes 1 ml
Host Mouse
Clonality Monoclonal
Clone C5/D5
Search more