Anti-MiTF antibody [C5] - ChIP Grade
| Name | Anti-MiTF antibody [C5] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab12039 |
| Prices | $403.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | C5 |
| Applications | IHC-F IHC-P ICC/IF ICC/IF ChIP ICC/IF FC WB IP EMSA |
| Species Reactivities | Mouse, Rat, Chicken, Human |
| Antigen | N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells |
| Description | Mouse Monoclonal |
| Gene | MITF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-MiTF antibody [C5] - ChIP Grade (ab12039) at 1 µg/ml + Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/22803_MiTF-Primary-antibodies-ab12039-8.jpg)
Anti-MiTF antibody [C5] - ChIP Grade (ab12039) at 1 µg/ml + Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab12039 staining MiTF in embryonic day 4 chick eye tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with 4% paraformaldehyde, permeabilized using 1% saponin, blocked with 10% serum for 30 minutes at 37°C, then incubated with ab12039 at a 1/1000 dilution for 24 hours at 4deg;C. The secondary used was an Alexa-Fluor 488 conjugated mouse monoclonal, used at a 1/100 dilution. Counterstained with DAPI (blue).See Abreview
![Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/22805_MiTF-Primary-antibodies-ab12039-31.jpg)
Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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The E-box binding factors Max/Mnt, MITF, and USF1 act coordinately with FoxO to - The E-box binding factors Max/Mnt, MITF, and USF1 act coordinately with FoxO to
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{alpha}MSH and Cyclic AMP elevating agents control melanosome pH through a - {alpha}MSH and Cyclic AMP elevating agents control melanosome pH through a
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