Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody
| Name | Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab90444 |
| Prices | $376.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Rat, Rabbit, Dog, Human, Pig, Monkey |
| Antigen | Synthetic peptide derived from the sequence of human PDK1, conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | PDK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Mitochondrial Pyruvate dehydrogenase kinase 1 staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90444, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody (ab90444) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Rat brain lysateLane 3 : Mouse brain lysateLane 4 : Vero cell lysateLane 5 : ESK-4 cell lysateLane 6 : RK-13 cell lysateLane 7 : MDCK cell lysate