Anti-Mitofusin 1 antibody
| Name | Anti-Mitofusin 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab104274 |
| Prices | $359.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Rabbit, Horse, Bovine, Cat, Dog, Pig |
| Antigen | Synthetic peptide corresponding to a region within C terminal amino acids 683-732 (QVDITQKQLE EEIARLPKEI DQLEKIQNNS KLLRNKAVQL ENELENFTKQ) of Human Mitofusin 1 (NP_284941) |
| Description | Rabbit Polyclonal |
| Gene | MFN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab104274 staining Mitofusin 1 in the H9C2 cell line from Rat heart by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 5% Serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200 in PBS + 5% Goat serum) for 2 hours at 21°C. AnAlexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.See Abreview
Anti-Mitofusin 1 antibody (ab104274) at 1 µg/ml + Human placenta lysate at 10 µg
All lanes : Anti-Mitofusin 1 antibody (ab104274) at 1/1000 dilution (+ 5% non-fat milk for 16 hours at 4°C )Lane 1 : Whole tissue lysate of mouse heart (wild type)Lane 2 : Whole tissue lysate of mouse heart (wild type)Lane 3 : Whole tissue lysate of mouse heart (Knock-out)Lane 4 : Whole tissue lysate of mouse heart (Knock-out)Lysates/proteins at 20 µg per lane.SecondaryAn HRP-conjugated Goat anti-rabbit IgG at 1/5000 dilutiondeveloped using the ECL technique
Immunofluorescence analysis of HEK293 cells, staining Mitofusin 1 with ab104274. Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in 5% horse serum) for 1 hour at room temperature. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.See Abreview
Product References
Pid1 induces insulin resistance in both human and mouse skeletal muscle during - Pid1 induces insulin resistance in both human and mouse skeletal muscle during
Bonala S, McFarlane C, Ang J, Lim R, Lee M, Chua H, Lokireddy S, Sreekanth P, Leow MK, Meng KC, Shyong TE, Lee YS, Gluckman PD, Sharma M, Kambadur R. Mol Endocrinol. 2013 Sep;27(9):1518-35.
Defective mitochondrial dynamics is an early event in skeletal muscle of an - Defective mitochondrial dynamics is an early event in skeletal muscle of an
Luo G, Yi J, Ma C, Xiao Y, Yi F, Yu T, Zhou J. PLoS One. 2013 Dec 6;8(12):e82112.
Mitofusin-2 maintains mitochondrial structure and contributes to stress-induced - Mitofusin-2 maintains mitochondrial structure and contributes to stress-induced
Papanicolaou KN, Khairallah RJ, Ngoh GA, Chikando A, Luptak I, O'Shea KM, Riley DD, Lugus JJ, Colucci WS, Lederer WJ, Stanley WC, Walsh K. Mol Cell Biol. 2011 Mar;31(6):1309-28.