Anti-Mitofusin 2 antibody
| Name | Anti-Mitofusin 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56889 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | IHC-F WB IHC-P IP ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Bovine, Human, Monkey |
| Antigen | Recombinant fragment: FKRQFVEHAS EKLQLVISYT GSNCSHQVQQ ELSGTFAHLC QQVDVTRENL EQEIAAMNKK IEVLDSLQSK AKLLRNKAGW LDSELNMFTH QYLQPSR, corresponding to amino acids 661-758 of Human Mitofusin 2 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | MFN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab56889 staining mitofusin 2 in MEF1 cells treated with nigericin Na+ salt (ab120494), by ICC/IF. Decrease in mitofusin 2 expression correlates with increased concentration of nigericin Na+ salt, as described in literature.The cells were incubated at 37°C for 3h in media containing different concentrations of ab120494 (nigericin Na+ salt) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab56889 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Mitofusin 2 antibody (ab56889) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.
ab56889 staining Mitofusin 2 in Mouse mitofusin 2-null cells transfected with Human mitofusin 2 by Immunocytochemistry/ Immunoflourescence. Cells were fixed in formaldehyde and permeabilized in 1% Triton X-100 prior to blocking in 10% goat serum for 1 hour at room temperature. The primary antibody was diluted 1/200 in 10% Goat serum with 5% BSA in PBST and incubated with the sample for 16 hours at 4°C. The secondary antibody was an Alexa Fluor® 488-conjugated Goat anti-Mouse polyclonal, diluted 1/500. DAPI was used for the blue nuclear counterstain.See Abreview
![Overlay histogram showing HEK293 cells stained with ab56889 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56889, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/22947_Mitofusin-2-Primary-antibodies-ab56889-6.jpg)
Overlay histogram showing HEK293 cells stained with ab56889 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56889, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
ab56889 staining mitodusin2 in MEF1 cells treated with valinomycin from Streptomyces fulvissimus (ab120852), by ICC/IF. Decrease in mitofusin2 expression with increased concentration of withaferin valinomycin from Streptomyces fulvissimus, as described in literature.The cells were incubated at 37°C for 3h in media containing different concentrations of ab120852 (valinomycin from Streptomyces fulvissimus) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab56889 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Anti-Mitofusin 2 antibody (ab56889) at 1/2000 dilution + Rat H9C2 cell lysate at 30 µgSecondaryHRP-conjugated Goat anti-mouse at 1/4000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Relationship between the expressions of mitofusin-2 and procollagen in - Relationship between the expressions of mitofusin-2 and procollagen in
Chen HY, Lu Y, Qi Y, Bai WP, Liao QP. Eur J Obstet Gynecol Reprod Biol. 2014 Mar;174:141-5. doi:
Ubiquitin is phosphorylated by PINK1 to activate parkin. - Ubiquitin is phosphorylated by PINK1 to activate parkin.
Koyano F, Okatsu K, Kosako H, Tamura Y, Go E, Kimura M, Kimura Y, Tsuchiya H, Yoshihara H, Hirokawa T, Endo T, Fon EA, Trempe JF, Saeki Y, Tanaka K, Matsuda N. Nature. 2014 Jun 5;510(7503):162-6.
Cellular consequences of oxidative stress in riboflavin responsive multiple - Cellular consequences of oxidative stress in riboflavin responsive multiple
Cornelius N, Corydon TJ, Gregersen N, Olsen RK. Hum Mol Genet. 2014 Aug 15;23(16):4285-301.
Mitochondrial fusion protein MFN2 interacts with the mitostatin-related protein - Mitochondrial fusion protein MFN2 interacts with the mitostatin-related protein
Vadnais ML, Lin AM, Gerton GL. Cilia. 2014 May 29;3:5.
A mitochondrial origin for frontotemporal dementia and amyotrophic lateral - A mitochondrial origin for frontotemporal dementia and amyotrophic lateral
Bannwarth S, Ait-El-Mkadem S, Chaussenot A, Genin EC, Lacas-Gervais S, Fragaki K, Berg-Alonso L, Kageyama Y, Serre V, Moore DG, Verschueren A, Rouzier C, Le Ber I, Auge G, Cochaud C, Lespinasse F, N'Guyen K, de Septenville A, Brice A, Yu-Wai-Man P, Sesaki H, Pouget J, Paquis-Flucklinger V. Brain. 2014 Aug;137(Pt 8):2329-45.
MUL1 acts in parallel to the PINK1/parkin pathway in regulating mitofusin and - MUL1 acts in parallel to the PINK1/parkin pathway in regulating mitofusin and
Yun J, Puri R, Yang H, Lizzio MA, Wu C, Sheng ZH, Guo M. Elife. 2014 Jun 4;3:e01958.
CDK5-dependent inhibitory phosphorylation of Drp1 during neuronal maturation. - CDK5-dependent inhibitory phosphorylation of Drp1 during neuronal maturation.
Cho B, Cho HM, Kim HJ, Jeong J, Park SK, Hwang EM, Park JY, Kim WR, Kim H, Sun W. Exp Mol Med. 2014 Jul 11;46:e105.
Endoplasmic reticulum stress mediates amyloid beta neurotoxicity via - Endoplasmic reticulum stress mediates amyloid beta neurotoxicity via
Barbero-Camps E, Fernandez A, Baulies A, Martinez L, Fernandez-Checa JC, Colell A. Am J Pathol. 2014 Jul;184(7):2066-81.
The deubiquitinase USP15 antagonizes Parkin-mediated mitochondrial ubiquitination - The deubiquitinase USP15 antagonizes Parkin-mediated mitochondrial ubiquitination
Cornelissen T, Haddad D, Wauters F, Van Humbeeck C, Mandemakers W, Koentjoro B, Sue C, Gevaert K, De Strooper B, Verstreken P, Vandenberghe W. Hum Mol Genet. 2014 Oct 1;23(19):5227-42.
Human cytomegalovirus inhibits apoptosis by proteasome-mediated degradation of - Human cytomegalovirus inhibits apoptosis by proteasome-mediated degradation of
Zhang A, Hildreth RL, Colberg-Poley AM. J Virol. 2013 May;87(10):5657-68.